TCR Vβ3 Hamster anti-Mouse, Brilliant Violet 711, Clone: KJ25, BD Optibuild™

Armenian Hamster Monoclonal Antibody

Manufacturer:  BD Biosciences 743416

Catalog No. BDB743416

Description

The KJ25 antibody specifically reacts with the V β 3 T-cell Receptor (TCR) of strains having the a (e.g., C57BR, SJL), b (e.g., AKR, CBA/Ca, C57BL, DBA/1), and c (e.g., RIII) haplotypes of the Tcrb gene complex. V β 3 TCR-bearing T lymphocytes are clonally eliminated either completely or partially in mice expressing superantigens encoded by the Mtv-1 ( Mls-4[a], Mls[c] ), Mtv-3 ( Mls[c] ), Mtv-6 ( Mls-3[a], Mls[c] ), Mtv-13 ( Mls-2[a], Mls[c] ), Mtv-27, Mtv-44, and/or Mtv-MAI endogenous proviruses (e.g., A, BALB/c, CBA/J, C3H/He, DBA/2, NZB, NZW). V β 3 TCR-bearing T cells are activated by the superantigenic Staphylococcal Enterotoxins A and B. Activation or elimination of V β 3 TCR-expressing T cells by these determinants is partially dependent upon presentation by I-E. This hamster mAb to a mouse leukocyte antigen does not cross-react with rat leukocytes.

The antibody was conjugated to BD Horizon™ BV711 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 711nm. BD Horizon BV711 can be excited by the violet laser and detected in a filter used to detect Cy™5.5 / Alexa Fluor™ 700-like dyes (eg, 712/20nm filter). Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors. However, the spillover can be corrected through compensation as with any other dye combination. BV711 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 711 nm. This dye offers a very bright choice for the violet laser. Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors. BV711 will also have moderate spillover into the BD Horizon Brilliant™ Violet 786 detector.

Specifications

• Antigen: TCR Vβ3
• Concentration: 0.2mg/mL
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Gene Alias: TCR V beta 3, TCR Vβ3
• Immunogen: αβ TCR purified from mouse T-cell hybridoma 2B4.6
• Purification Method: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV711 under optimal conditions that minimize unconjugated dye and antibody.
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Murine

• Clone: KJ25
• Conjugate: Brilliant Violet 711
• Format: Affinity Purified
• Host Species: Armenian Hamster
• Isotype: Armenian Hamster IgG₂, κ
• Quantity: 50μg
• Storage Requirements: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
• Monoclonal or Polyclonal: Monoclonal