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TCR Vβ8 Mouse anti-Human, Brilliant Violet 421, Clone: JR2, BD Optibuild™

Mouse Monoclonal Antibody

Manufacturer:  BD Biosciences 742412

Catalog No. BDB742412


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Description

Description

The JR2 monoclonal antibody specifically recognizes the -chain variable region (V 8) of the T-cell antigen receptor expressed on 0.5% to 3% of peripheral blood T cells. This antibody has been further characterized as having specificity to a major subset of the BV8 region as determined in the TCR Workshop of July 1995. Staphylococcal enterotoxin E has been shown to specifically stimulate T cells bearing V 8 (BV8).

The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407nm and Em Max at 421nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates. BV421 is a polymer-based dye excited by the violet laser and is one of the brightest fluorochromes offered by BD Biosciences. Conjugates are typically 10 times brighter than Pacific Blue™ conjugates and are often as bright as or brighter than PE conjugates. Due to nearly identical excitation and emission properties but different spillover characteristics, BD Horizon BV421, Pacific Blue™, and BD Horizon™ V450 cannot be used simultaneously.
Specifications

Specifications

TCR Vβ8
0.2mg/mL
Aqueous buffered solution containing ≤0.09% sodium azide.
BV8, TCR Vβ8, TCR V beta 8, TCR Vb8
Mouse IgG2b, κ
50μg
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Monoclonal
JR2
Brilliant Violet 421
Affinity Purified
Murine
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV421 under optimal conditions that minimize unconjugated dye and antibody.
RUO
Primary
Human
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