Invitrogen™ TCR V beta 6 Monoclonal Antibody (RR4-7), APC, eBioscience™

Catalog No.	Quantity
50-245-691	100 μg

Catalog No. 50-245-691 Supplier Invitrogen™ Supplier No. 17579582

Description

Rat Monoclonal Antibody

This RR4-7 monoclonal antibody reacts with mouse V beta 6 TCR. Composed of an a and b chain, TCR specificity is typically determined by Va, Ja, Vb, Db, and Jb gene rearrangement. The RR4-7 antibody recognizes the V beta 6 chain on T cells from mouse strains with the a (e.g., C57BR and SJL) and b haplotypes (e.g., BALB/c, C3H, and DBA/1). Reports indicate that strains expressing the Mls-1a antigen, particularly in the presence of MHC Class II I-E, possess fewer V beta 6 TCR+ T cells. The RR4-7 antibody has been reported to have functional activity in vitro and in vivo. Applications Reported: This RR4-7 antibody has been reported for use in flow cytometric analysis. Applications Tested: This RR4-7 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser. Filtration: 0.2 μm post-manufacturing filtered.

The ability of T cell receptors (TCR) to discriminate foreign from self-peptides presented by major histocompatibility complex (MHC) class II molecules is essential for an effective adaptive immune response. TCR recognition of self-peptides has been linked to autoimmune disease. Mutant self-peptides have been associated with tumors. Engagement of TCRs by a family of bacterial toxins know as superantigens has been responsible for toxic shock syndrome. Autoantibodies to V beta segments of T cell receptors have been isolated from patients with rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). The autoantibodies block TH1-mediated inflammatory autodestructive reactions and are believed to be a method by which the immune system compensates for disease. Most human T cells express the TCR alpha-beta and either CD4 or CD8 molecule (single positive, SP). A small number of T cells lack both CD4 and CD8 (double negative, DN). Increased percentages of alpha-beta DN T cells have been identified in some autoimmune and immunodeficiency disorders. Gamma-delta T cells are primarily found within the epithelium. They show less TCR diversity and recognize antigens differently than alpha-beta T cells. Subsets of gamma-delta T cells have shown antitumor and immunoregulatory activity.

Specifications

• Antigen: TCR V beta 6
• Applications: Flow Cytometry
• Classification: Monoclonal
• Clone: RR4-7
• Concentration: 0.2 mg/mL
• Conjugate: APC
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Alias: TCR V beta6; TCRV beta 6; TCRV beta6; Vb6; Vbeta6
• Host Species: Rat
• Purification Method: Affinity chromatography
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Mouse
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG2b κ