Invitrogen™ TCR V beta 9 Monoclonal Antibody (MR10-2), eFluor™ 450, eBioscience™

Catalog No.	Quantity
50-246-015	25 μg

Catalog No. 50-246-015 Supplier Invitrogen™ Supplier No. 48582380

Description

Mouse Monoclonal Antibody

Description: This MR10-2 monoclonal antibody reacts with the mouse T-cell receptor (TCR) V beta 9 chain. Composed of an a and β chain, TCR specificity is typically determined by Va, Ja, Vb, Db, and Jb gene rearrangement. The MR10-2 antibody recognizes the V beta 9 chain on T cells from mouse strains with the b haplotype, including BALB/c, CBA/Ca, C3H/He, and C57BL. This gene is deleted in mice bearing the a (e.g., C57BR, C57L, SJL, and SWR) or c (e.g, RIII) haplotypes. T cells expressing V beta 9 are also deleted in mice expressing the minor lymphocyte stimulating (MIs)-1a antigen. The MR10-2 antibody has been reported to activate V beta 9+ T cells. Applications Reported: Anti-Mouse V beta 9 TCR eFluor® 450 has been reported for use in flow cytometric analysis. Applications Tested: Anti-Mouse V beta 9 TCR eFluor® 450 has been tested by flow cytometric analysis of mouse lymph node. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome. Ex...

The ability of T cell receptors (TCR) to discriminate foreign from self-peptides presented by major histocompatibility complex (MHC) class II molecules is essential for an effective adaptive immune response. TCR recognition of self-peptides has been linked to autoimmune disease. Mutant self-peptides have been associated with tumors. Engagement of TCRs by a family of bacterial toxins know as superantigens has been responsible for toxic shock syndrome. Autoantibodies to V beta segments of T cell receptors have been isolated from patients with rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). The autoantibodies block TH1-mediated inflammatory autodestructive reactions and are believed to be a method by which the immune system compensates for disease. Most human T cells express the TCR alpha-beta and either CD4 or CD8 molecule (single positive, SP). A small number of T cells lack both CD4 and CD8 (double negative, DN). Increased percentages of alpha-beta DN T cells have been identified in some autoimmune and immunodeficiency disorders. Gamma-delta T cells are primarily found within the epithelium. They show less TCR diversity and recognize antigens differently than alpha-beta T cells. Subsets of gamma-delta T cells have shown antitumor and immunoregulatory activity.

Specifications

• Antigen: TCR V beta 9
• Applications: Flow Cytometry
• Classification: Monoclonal
• Clone: MR10-2
• Concentration: 0.2 mg/mL
• Conjugate: eFluor 450
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Alias: TCR V beta9; TCRV beta 9; TCRV beta9; Vb9; Vbeta9
• Host Species: Mouse
• Purification Method: Affinity chromatography
• Quantity: 25 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Mouse
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG1 κ