Invitrogen™ TCR V delta 4 Monoclonal Antibody (GL2), eFluor™ 660, eBioscience™

Armenian Hamster Monoclonal Antibody

Supplier:  Invitrogen™ 50570280

Catalog No. 50-570-280

Description

Description: This GL2 monoclonal antibody reacts with the mouse V delta 4 T cell receptor (TCR). The gamma delta TCR is expressed by a small subset of T lymphocytes in the thymus, intestinal epithelium, spleen, lymph node, and epidermis. Applications Reported: This GL2 antibody has been reported for use in flow cytometric analysis. Applications Tested: This GL2 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.06 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser. Filtration: 0.2 μm post-manufacturing filtered.

The ability of T cell receptors (TCR) to discriminate foreign from self-peptides presented by major histocompatibility complex (MHC) class II molecules is essential for an effective adaptive immune response. TCR recognition of self-peptides has been linked to autoimmune disease. Mutant self-peptides have been associated with tumors. Engagement of TCRs by a family of bacterial toxins know as superantigens has been responsible for toxic shock syndrome. Autoantibodies to V beta segments of T cell receptors have been isolated from patients with rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). The autoantibodies block TH1-mediated inflammatory autodestructive reactions and are believed to be a method by which the immune system compensates for disease. Most human T cells express the TCR alpha-beta and either CD4 or CD8 molecule (single positive, SP). A small number of T cells lack both CD4 and CD8 (double negative, DN). Increased percentages of alpha-beta DN T cells have been identified in some autoimmune and immunodeficiency disorders. Gamma-delta T cells are primarily found within the epithelium. They show less TCR diversity and recognize antigens differently than alpha-beta T cells. Subsets of gamma-delta T cells have shown antitumor and immunoregulatory activity.

Specifications

• Antigen: TCR V delta 4
• Classification: Monoclonal
• Concentration: 0.2 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Host Species: Armenian Hamster
• Quantity: 25 μg
• Primary or Secondary: Primary
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: Flow Cytometry
• Clone: GL2
• Conjugate: eFluor 660
• Gene Alias: TCR V delta4; TCRV delta 4; TCRV delta4; Vd4; Vdelta4
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG