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Invitrogen™ TdT Monoclonal Antibody (19-3), PE, eBioscience™
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Catalog No. 5010903
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100 μg
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Catalog No. Quantity
50-109-03 100 μg
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Catalog No. 50-109-03 Supplier Invitrogen™ Supplier No. 12584682
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Description

Mouse Monoclonal Antibody

Description: This 19-3 monoclonal antibody recognizes human and mouse terminal-deoxynucleotidyl transferase (TdT), a 60 kDa polymerase responsible for the template-independent addition of N-nucleotides at gene segment junctions of developing lymphocytes. Expression is found in the nucleus of immature lymphocytes but not in mature lymphocytes or non-lymphoid cells. Tdt plays a critical role during TCR and Ig gene rearrangement. Regulation of expression in the mouse thymus correlates with T cell selection; decreased or absent after positive selection. Additionally Tdt has been shown to be present in lymphoma or lymphoblastic leukemia cells. Applications Reported: This 19-3 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This 19-3 antibody has been tested by intracellular staining using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5521-00) followed by flow cytometric analysis of mouse thymocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 μm post-manu...

Terminal Deoxynucleotidyl Transferase (TdT) is a DNA polymerase located in the cell nucleus which catalyses the polymerization of deoxynucleotides at the 3' hydroxyl ends of oligo or polydeoxynucleotide initiators and functions without a template. TdT is considered to be a highly specific marker for the diagnosis and classification of acute lymphoblastic lymphoma/leuksemias. The determination of TdT expression is most valuable when it is different to differentiate histologically between lymphoblastic lymphoma and Burkitt's lymphoma.
TRUSTED_SUSTAINABILITY

Specifications

Antigen TdT
Applications Flow Cytometry
Classification Monoclonal
Clone 19-3
Concentration 0.2 mg/mL
Conjugate PE
Formulation PBS with 0.09% sodium azide; pH 7.2
Gene DNTT
Gene Accession No. P04053, P09838
Gene Alias Deoxynucleotidyltransferase terminal; deoxynucleotidyltransferase, terminal; DNA nucleotidylexotransferase; Dntt; Nucleosidetriphosphate DNA Deoxynucleotidylexotransferase; nucleosidetriphosphate:DNA deoxynucleotidylexotransferase; TDT; Terminal addition enzyme; terminal deoxynucleotidyl transferase; terminal deoxynucleotidyltransferase; terminal deoxyribonucleotidyltransferase; terminal transferase
Gene Symbols DNTT
Host Species Mouse
Purification Method Affinity chromatography
Quantity 100 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 1791, 21673
Target Species Human, Mouse
Content And Storage 4°C, store in dark, DO NOT FREEZE!
Product Type Antibody
Form Liquid
Isotype IgG2b κ
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Safety and Handling

WARNING: Cancer and Reproductive Harm - www.P65Warnings.ca.gov
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