Invitrogen™ TECK Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ 500P134BT25UG

Catalog No. 500P134BT25

Description

AA Sequence of recombinant protein: QGVFEDCCLA YHYPIGWAVL RRAWTYRIQE VSGSCNLPAA IFYLPKRHRK VCGNPKSREV QRAMKLLDAR NKVFAKLHHN MQTFQAGPHA VKKLSSGNSK LSSSKFSNPI SSSKRNVSLL ISANSGL. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human TECK (CCL25). Anti-Human TECK (CCL25)-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect Human TECK (CCL25) by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human TECK (CCL25) (500-P134) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human TECK (CCL25). Western Blot: To detect hTECK by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hTECK is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P134BT-1MG will be provided as 2 x 500 μg

Chemokines are likely to play an important role in regulating the trafficking of developing T cells within the thymus. Chemokine C-C thymus expressed chemokine (TECK), also designated chemokine ligand 25 (CCL25), small induc-ible cytokine A25, chemokine b-15 or CK b-15, is expressed predominantly in thymic dendritic cells, thymic epithelial cells and in the small intestine. TECK, a CCR9 ligand, has suppressive activity against immature subsets of myeloid progenitors which have been stimulated to proliferate by multiple growth factors. TECK delivers signals through CCR9, which is important for the nav-igation of developing thymocytes. Bone marrow pre-pro-B cells and cells capable of generating pro-B colonies in the presence of interleukin-7 and Flt-3 ligand migrate to TECK, a response lost in later stages of B cell development.

Specifications

• Antigen: TECK
• Classification: Polyclonal
• Conjugate: Biotin
• Gene: CCL25
• Gene Alias: A130072A22Rik; AI852536; C-C motif chemokine 25; C-C motif chemokine ligand 25; CCL25; chemokine (C-C motif) ligand 25; chemokine TECK; Ck beta-15; CKb15; Scya25; SCYA25TECKvar; small inducible chemokine 25; small inducible cytokine A25; small inducible cytokine subfamily A (Cys-Cys), member 25; small-inducible cytokine A25; Teck; TECKMGC150327; TECKvar; thymus expressed chemokine; thymus-expressed chemokine
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 6370
• Content And Storage: -20°C
• Form: Lyophilized

• Applications: ELISA, Western Blot
• Concentration: 0.1-1.0 mg/mL
• Formulation: PBS with no preservative
• Gene Accession No.: O15444
• Gene Symbols: CCL25
• Immunogen: E.coli-derived, 14.2 kDa Recombinant Human TECK (CCL25).
• Quantity: 25 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody