Invitrogen™ Human TEK (TIE2) [Y897S], GST Tag Recombinant Protein

Description

Recombinant Protein

For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 μg, 10 μg and 20 μg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 μL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase. This TEK (TIE2) Y897S recombinant human protein (771-end) was expressed in insect cells. TEK (TIE2) Y897S recombinant human protein is also available in our SelectScreen™ Kinase Profiling Service. : Validated for use with the Z'-LYTE™ assay: Available in multiple pack sizes for convenient use: Also available in our SelectScreen Kinase Profiling Service Protein Form: Recombinant, Active, Catalytic The gene accession number for TEK (TIE2) [Y897S] is NP_000450.2.

TEK (TIE2) Y897S receptor tyrosine kinase is expressed principally on vascular endothelium. Disrupting TEK Y897S function in mice results in embryonic lethality with defects in embryonic vasculature, suggesting a role in blood vessel maturation and maintenance. Angiopoietin-1 is a secreted growth factor that binds to and activates the TEK receptor tyrosine kinase. SHP2 and GRB2 are recruited to the activated TEK kinase domain and are part of the cellular responses that mediate TEK function. TEK expression is upregulated in the endothelium of vascular 'hot spots' in human breast cancer specimens. However, TEK is also overexpressed in areas of active angiogenesis in normal tissues.

Specifications

• Accession Number: Q02763
• Concentration: See Label
• For Use With (Application): Kinase Assay
• Formulation: 50mM tris HCl with 0.25mM DTT, 0.1mM EDTA, 10mM glutathione, 25% glycerol, 150mM NaCl, 0.1mM PMSF and no preservative; pH 7.5
• Gene ID (Entrez): 7010
• Molecular Weight (g/mol): 66.7 kDa
• Name: Human TEK (TIE2) [Y897S], GST Tag
• pH Range: 7.5
• Purification Method: Purified
• Quantity: 10 μg
• Storage Requirements: -80°C, Avoid Freeze/Thaw Cycles
• Regulatory Status: RUO
• Gene Alias: TIE2
• Common Name: TIE2 (TEK) (Y897S Mutant)
• Gene Symbol: TEK
• KinaseFamily: TIE Kinase Family
• Protein Length: 771-end
• Product Type: Protein
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Protein Tag: GST-tag
• Sequence: 771-end
• Content And Storage: -80°C, Avoid Freeze/Thaw Cycles
• Shipping Condition: Dry Ice
• Expression System: Insect cells
• Protein Family: Kinases & Inhibitors
• Protein Form: Recombinant, Active, Catalytic, Mutant
• Form: Liquid