MilliporeSigma™ TET1, Rat, Unlabeled, Clone: 5D6,

Catalog No.	Quantity	Conjugate
MABE1144MI	100 μg	Unconjugated

Catalog No. MABE1144MI Supplier MilliporeSigma™ Supplier No. MABE1144

Description

Rat Monoclonal Antibody

Methylcytosine dioxygenase TET1 (EC 1.14.11.n2; UniProt Q3URK3; also known as CXXC-type zinc finger protein 6, Ten-eleven translocation 1 gene protein homolog) is encoded by the Tet1 (also know as Cxxc6, Kiaa1676) gene (Gene ID 52463) in murine species. TET (ten-eleven translocation) proteins (TET1, TET2, and TET3) oxidize 5-methylcytosine to 5-hydroxymethylcytosine, 5-formylcytosine, and 5-carboxylcytosine, providing a means for active epigenetic DNA demethylation in mammals. TET proteins also modify genomic thymine residues to 5-hydroxyuracil. TET proteins depend on Fe(II) and 2-oxoglutarate as cofactors for activity, and 2-oxoglutarate is known to inhibit their activity, while ascorbate is shown to stimulate TET-mediated cytosine oxidation. In addition, post-translational modifications (PTMs), such as phosphorylation and O-GlcNAcylation, also play a role in regulating the activity of TET proteins. TET1 and TET2 are found highly expressed in mouse embryonic stem cells (mESCs) and TET3 is known to be upregulated in oocytes and oxidize the silenced paternal pronuclear DNA. High levels of TET proteins and genomic 5-hydroxymethylcytosine in neuronal tissues are also reported.

Specifications

• Antigen: TET1
• Applications: Immunocytochemistry, Immunoprecipitation, Western Blot
• Classification: Monoclonal
• Clone: 5D6
• Concentration: Please refer to lot specific datasheet.
• Conjugate: Unconjugated
• Formulation: Purified rat monoclonal IgG2aκ antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.
• Gene: Tet1, Cxxc6, Kiaa1676
• Gene Accession No.: Q3URK3
• Host Species: Rat
• Immunogen: His-tagged recombinant fragment from the region between DNA-binding and 2-oxoglutarate-binding domains of mouse TET1.
• Purification Method: Protein G Purified
• Quantity: 100 μg
• Regulatory Status: RUO
• Research Discipline: Epigenetics & Nuclear Function
• Primary or Secondary: Primary
• Gene ID (Entrez): NP_081660
• Test Specificity: Clone 5D6 detected exogenously expressed TET1, but not TET2 or TET3, GST fusion protein (Bauer, C., et al. (2015). J. Biol. Chem. 290(8):4801-4812).
• Target Species: Mouse
• Content And Storage: Stable for 1 year at 2°-8°C from date of receipt.
• Form: Purified
• Isotype: IgG2a κ

For Research Use Only. Not for use in diagnostic procedures.