TET2 Polyclonal Antibody, Rockland™

Catalog No.	Quantity
50-199-0140	25 μL
50-199-0139	100 μL

Catalog No. 50-199-0140 Supplier Rockland Immunochemicals Supplier No. 100401N93S

Description

Rabbit Polyclonal Antibody

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. It is directed against, and shows specific reactivity for human Tet2 protein. Cross reactivity with Tet1 and Tet3 has not been determined.

TET2 is a dioxygenase that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5-hydromethylcytosine (5hmC) and plays a key role in active DNA demethylation. TET2 has a preference for 5-hydroxymethylcytosine in CpG motifs. It mediates subsequent conversion of 5hmC into 5-formylcytosine (5fC) and conversion of 5fC to 5-carboyxlcytosine (5caC). Conversion of 5mC into 5hmC, 5fC, and 5caC probably constitutes the first step in cytonsine demethylation. In addition to TET2’s role in DNA demethylation, it is also involved in the recruitment of the O-GlcNAc transferase OGT to CpG-rich transcription start sites of active genes, thereby promoting histone H2B GlcNAcylation by OGT. Mutations in the genes can result in Polycythemia vera and Myelodysplastic syndrome.

Specifications

• Antigen: TET2
• Applications: ELISA, Western Blot
• Classification: Polyclonal
• Concentration: 72.5 mg/mL
• Conjugate: Unconjugated
• Formulation: 0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
• Gene: TET2
• Gene Accession No.: Q6N021
• Gene Alias: Ayu17-449; E130014J05Rik; FLJ20032; KIAA1546; MDS; Methylcytosine dioxygenase TET2; MGC125715; mKIAA1546; Nbla00191; OTTHUMP00000161869; probable methylcytosine dioxygenase TET2; Protein Ayu17 449; Protein Ayu17-449; RGD1311625; Tet 2; tet methylcytosine dioxygenase 2; tet oncogene 2; tet oncogene family member 2; Tet2
• Gene Symbols: TET2
• Host Species: Rabbit
• Immunogen: TET2 antibody was prepared from whole rabbit serum produced by repeated immunizations with a human TET-2 domain containing the N-terminal 156 amino acids of the protein.
• Quantity: 25 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 54790
• Target Species: Human
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Product Type: Antibody
• Form: Liquid