Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More
  1. Home
  2. Products
  3. Antibodies
  4. Primary Antibodies
  5. All Primary Antibodies
  6. MABE1132MI

MilliporeSigma™ TET2, Rat, Unlabeled, Clone: 9F7,

Catalog No. MABE1132MI Shop All MilliporeSigma Products
Change view
Click to view available options
Quantity:
100 μg
Conjugate:
Unconjugated
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Quantity Conjugate
MABE1132MI 100 μg Unconjugated
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
Catalog No. MABE1132MI Supplier MilliporeSigma™ Supplier No. MABE1132
Add to Cart
Add to Cart

Description

Rat Monoclonal Antibody

Methylcytosine dioxygenase TET2 (EC 1.14.11.n2; UniProt Q4JK59; also known as Protein Ayu17-449) is encoded by the Tet2 (also known as Kiaa1546) gene (Gene ID 214133) in murine species. TET (ten-eleven translocation) proteins (TET1, TET2, and TET3) oxidize 5-methylcytosine to 5-hydroxymethylcytosine, 5-formylcytosine, and 5-carboxylcytosine, providing a means for active epigenetic DNA demethylation in mammals. TET proteins also modify genomic thymine residues to 5-hydroxyuracil. TET proteins depend on Fe(II) and 2-oxoglutarate as cofactors for activity, and 2-oxoglutarate is known to inhibit their activity, while ascorbate is shown to stimulate TET-mediated cytosine oxidation. In addition, post-translational modifications (PTMs), such as phosphorylation and O-GlcNAcylation, also play a role in regulating the activity of TET proteins. TET1 and TET2 are found highly expressed in mouse embryonic stem cells (mESCs) and TET3 is known to be upregulated in oocytes and oxidize the silenced paternal pronuclear DNA. High levels of TET proteins and genomic 5-hydroxymethylcytosine in neuronal tissues are also reported.
TRUSTED_SUSTAINABILITY

Specifications

Antigen TET2
Applications Immunocytochemistry, Immunoprecipitation, Western Blot
Classification Monoclonal
Clone 9F7
Concentration Please refer to lot specific datasheet.
Conjugate Unconjugated
Formulation Purified rat monoclonal IgG2aκ antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.
Gene Tet2, Kiaa1546
Gene Accession No. Q4JK59
Host Species Rat
Immunogen His-tagged recombinant fragment from the region between DNA-binding and 2-oxoglutarate-binding domains of mouse TET2.
Purification Method Protein G Purified
Quantity 100 μg
Regulatory Status RUO
Research Discipline Epigenetics & Nuclear Function
Primary or Secondary Primary
Gene ID (Entrez) NP_001035490
Test Specificity Clone 9F7 detected exogenously expressed TET2, but not TET1 or TET3, GST fusion (Bauer, C., et al. (2015). J. Biol. Chem. 290(8):4801-4812). Epitope region is present in all three murine TET2 spliced isoforms reported by UniProt (Q4JK59).
Target Species Mouse
Content And Storage Stable for 1 year at 2°-8°C from date of receipt.
Form Purified
Isotype IgG2a κ
Show More Show Less

For Research Use Only. Not for use in diagnostic procedures.

Product Title
Select an issue

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.