Tetracycline, MP Biomedicals™

Description

Tetracyline is used as an antibiotic, in tetracycline controlled gene expression systems, and to select tetracyline resistant colonies.

• Tetracyline inhibits gram-positive, gram-negative and mycoplasma.
• It is used in tetracycline controlled gene expression systems (gene switches) such as the tet-on and tet-off systems.
• It is also used to select tetracyline resistant colonies in cell culture.

Mode of Action: Tetracycline passively diffuses through proin channels in the cell membrane, binding to 30S ribosomes and inhibits protein synthesis by preventing access of aminoacyl tRNA to the acceptor site on the mRNA-ribosome complex. It also binds to the bacterial 50S ribosomal subunit, altering the membrane and causing intracellular components to leak from bacterial cells. The inhibitory effects can be reversed by washing, suggesting that it is the reversibly bound antibiotic, and not the irreversibly bound drug, that is responsible for antibacterial action.

Mode of Resistance: The effects are inactivated via a loss of cell wall permeability.

Antimicrobial spectrum: Includes a wide range of antimicrobial activity against gram-positive and gram-negative bacteria.

Specifications

Chemical Identifiers

• CAS: 60-54-8
• Molecular Formula: C22H24N2O8
• Molecular Weight (g/mol): 444.44
• MDL Number: MFCD00151232
• InChI Key: JYHCQVWYCGHXGP-BPPSBWQWSA-N
• Synonym: Abramycin, Tetraverine
• PubChem CID: 54675776
• IUPAC Name: (2Z,4S,4aS,5aS,6S,12aS)-2-[amino(hydroxy)methylidene]-4-(dimethylamino)-6,10,11,12a-tetrahydroxy-6-methyl-1,2,3,4,4a,5,5a,6,12,12a-decahydrotetracene-1,3,12-trione
• SMILES: CN(C)[C@H]1[C@@H]2C[C@H]3C(=C(O)C4=C(O)C=CC=C4[C@@]3(C)O)C(=O)[C@]2(O)C(=O)\C(=C(\N)O)C1=O

• Type: Tetracyline Free Base
• Physical Form: Powder
• Absorbance: λmax (0.1N HCl) 217 to 220, 268 to 271, 355 to 356 nm
• pH: 3.0 to 7.0 (saturated soln.)
• Melting Point: 417°F (Decomposition)
• Source: Streptomyces spp.
• Potency: ≥900 μg/mg
• Color: Yellow
• Concentration: 10 μg/mL
• Quantity: 100 g
• Optical Rotation: [α]20/D −230 ± 5°, c = 1% in methanol
• Assay Percent Range: 0.944
• Solubility Information: 50 to 100 mg/mL at 72°F
• Formula Weight: 444.4
• Chemical Name or Material: Tetracyline Free Base

Safety and Handling

storageNote1	Protect from light. Store under nitrogen.
Recommended Storage	Store at 0°C.

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use.