Tex19.1, Mouse anti-Mouse, Clone: 7TEX-1F11, Millipore Sigma™

Catalog No.	Quantity
MABC1596100	100 μg
MABC159625	25 μg

Catalog No. MABC1596100 Supplier MilliporeSigma Supplier No. MABC1596100UG

Description

Mouse Monoclonal Antibody

Anti-Tex19.1, clone 7TEX-1F11, Cat. No. MABC1596, is a mouse monoclonal antibody that detects Testis-expressed protein 19.1 and has been tested for use in ELISA, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.

Testis-expressed protein 19.1 (UniProt: Q99MV2; also known as mTex19.1, Testis-expressed protein 19A) is encoded by the Tex19.1 (also known as Tex19, Tex19a) gene (Gene ID: 73679) in murine species. Tex19.1 resides in the germline to repress transposable genetic elements and maintain genomic stability. It is expressed in testis, placenta, ovary, and in pluripotent stem cells. In testis its expression is highest in mitotic spermatogonia and decreases as spermatocytes progress through meiosis. Tex19.1 expression is reported to be down-regulated by Deleted in azoospermia-like (DAZL) protein, which binds to 3'UTR of Tex19.1 mRNAs and repress its translation. Tex19.1 is required during spermatogenesis and placenta development where it participates in the repression of retrotransposable elements and prevents their mobilization. Tex19.1, along with its paralog, Tex19.2, is shown to collaborate with the Piwi-interacting RNA (piRNA) pathway, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins. Embryos with mutations in Tex19.1 gene exhibit intra-uterine growth retardation and have small placentas due to a reduction in the number of spongiotrophoblast, glycogen trophoblast and sinusoidal trophoblast giant cells. Loss of Tex19.1 is shown to cause growth defect and early post-natal lethality. Male animals with deficiency of both Tex19.1 and Tex19.2 display impaired spermatogenesis, small testes, and are infertility.

Specifications

• Antigen: Tex19.1
• Applications: ELISA, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
• Classification: Monoclonal
• Clone: 7TEX-1F11
• Conjugate: Unconjugated
• Dilution: Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected Tex19.1 in embryonic mouse brain and embryonic mouse gut tissue sections.Immunocytochemistry Analysis: A representative lot detected Tex19.1 in Immunocytochemistry applications (Tarabay, Y., et. al. (2013). Hum Reprod. 28(8):2201-14).ELISA Analysis: A representative lot detected Tex19.1 in ELISA applications (Tarabay, Y., et. al. (2013). Hum Reprod. 28(8):2201-14).Immunoprecipitation Analysis: A representative lot immunoprecipitated Tex19.1 in Immunoprecipitation applications (Tarabay, Y., et. al. (2017). J Cell Sci. 130(8):1463-1474).Western Blotting Analysis: A representative lot detected Tex19.1 in Western Blotting applications (Tarabay, Y., et. al. (2013). Hum Reprod. 28(8):2201-14;Tarabay, Y., et. al. (2017). J Cell Sci. 130(8):1463-1474).
• Formulation: Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
• Gene Alias: Testis-expressed protein 19.1;mTex19.1;Testis-expressed protein 19A
• Gene Symbols: Tex19.1;Tex19;Tex19a
• Host Species: Mouse
• Immunogen: His-tagged full-length recombinant mouse Testis-expressed protein 19.1 (Tex 19).
• Purification Method: Protein G purified
• Quantity: 100 μg
• Regulatory Status: RUO
• Research Discipline: Signaling
• Primary or Secondary: Primary
• Gene ID (Entrez): NP_082878
• Test Specificity: Clone 7TEX-1F11 detects murine Testis-expressed protein 19.1 (Tex 19).
• Target Species: Mouse
• Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
• Form: Purified
• Isotype: IgG2b κ