Invitrogen™ TFAM Recombinant Rabbit Monoclonal Antibody (JM32-44)

Rabbit Recombinant Monoclonal Antibody

Supplier:  Invitrogen™ MA532764

Catalog No. PIMA532764

Description

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Mitochondrial transcription factor A (TFAM) is a crucial protein encoded by nuclear genes, but it is transported from the cytoplasm to mitochondria where it plays a key role in the maintenance and expression of mitochondrial DNA (mtDNA). It regulates the replication and transcription of mtDNA, ensuring the proper functioning of mitochondrial processes. Structurally, TFAM contains a molecular region known as the LC3 interacting region (LIR) motif, which allows it to bind to autophagy proteins such as LC3. This interaction is essential for the autolysosomal pathway, helping to eliminate leaked mtDNA and limit inflammation. TFAM levels vary across tissues, with notable effects on mitochondrial function; high levels can repress mtDNA expression in certain tissues, such as skeletal muscle, and contribute to deficiencies in oxidative phosphorylation (OXPHOS). Conversely, in other tissues like the heart, increased mtDNA copy number maintains a balanced TFAM-to-mtDNA ratio, preserving OXPHOS capacity. TFAM is integral to the packaging, stability, and replication of the mitochondrial genome, and disruptions in TFAM can have severe effects, including implications in neurodegeneration and other diseases related to mtDNA stress.

Specifications

• Antigen: TFAM
• Classification: Recombinant Monoclonal
• Concentration: 1 mg/mL
• Formulation: TBS with 0.05% BSA, 40% Glycerol and 0.05% sodium azide; pH 7.4
• Gene Accession No.: Q00059
• Gene Symbols: Tfam
• Immunogen: Synthetic peptide within Human mtTFA aa 41-90.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG

• Applications: Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot, Immunocytochemistry, Western Blot
• Clone: JM32-44
• Conjugate: Unconjugated
• Gene: Tfam
• Gene Alias: AI661103; Hmgts; Mitochondrial transcription factor 1; mitochondrial transcription factor A; MTTF1; mtTFA; TCF6; TCF-6; TCF6L1; TCF6L2; TCF6L3; Testis-specific high mobility group protein; testis-specific HMG-box protein m-tsHMG; TFAM; Transcription factor 6; transcription factor 6-like 1; transcription factor 6-like 2; transcription factor 6-like 2 (mitochondrial transcription factor); transcription factor 6-like 3; transcription factor A, mitochondrial; tsHMG; TS-HMG
• Host Species: Rabbit
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 7019
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid