Invitrogen™ TGF alpha Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA184179

Catalog No. PA184179

Description

Heat-mediated antigen retrieval using a sodium citrate buffer (pH 6.0) is recommended for the staining of paraffin sections. This antibody may be used in Western Blotting applications under either reducing or non-reducing conditions. Reconstitute with 0.1 mL of distilled water. For long term storage the addition of 0.09% sodium azide is recommended (unless the product will be used for functional studies). Prior to reconstitution, store at 4ºC. After reconstitution, store undiluted at -20ºC, avoiding freeze/thaw cycles. Rabbit anti human TGFα recognizes tranforming growth factor alpha.

TGF-alpha is an EGF-related polypeptide growth factor that signals through the EGF receptor, and stimulates the proliferation of a wide range of epidermal and epithelial cells. It is produced by monocytes, keratinocytes, and various tumor cells. TGF-alpha induces anchorage-independence transformation in cultured cells. Human, murine and rat TGF-alpha are cross-species reactive. TGFalpha (aa 50) is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.

Specifications

• Antigen: TGF alpha
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: TGFA
• Gene Alias: EGF-like TGF; ETGF; I79_019067; LOC100009150; Protransforming growth factor alpha; RATTGFAA; TFGA; TGF a; TGF type 1; TGF a; TGFA; TGFAA; TGFalpha; TGF-alpha; TGFa; Transforming growth factor; Transforming growth factor alpha; transforming growth factor, alpha; transforming growth factor-alpha; wa1; wa-1; waved 1
• Host Species: Rabbit
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 7039
• Content And Storage: -20°C
• Form: Lyophilized

• Applications: ELISA, Functional Assay, Immunohistochemistry (Paraffin), Western Blot
• Concentration: 1 mg/mL
• Formulation: PBS with no preservative; pH 7.4
• Gene Accession No.: P01135
• Gene Symbols: TGFA
• Immunogen: Recombinant human TGF-alpha.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG