Invitrogen™ TGF beta-3 Monoclonal Antibody (20724)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA523812

Catalog No. PIMA523812

Description

In Western blots, less than 25% cross-reactivity with recombinant human (rh) TGF- beta 1.2 and rhTGF-beta 2 is observed, and less than 2% cross-reactivity with recombinant amphibian TGF-beta 5 and recombinant human TGF-beta 1 is observed. This antibody neutralizes the biological activity of TGF-beta 3 but not TGF-beta 1, TGF-beta 2, or TGF-beta 5. Reconstitute at 0.5 mg/mL in sterile PBS. Endoxin level is <0.10 EU per 1 μg of the antibody by the LAL method.

Transforming growth factor beta 3 (TGF beta 3) is the third member of the transforming growth factor family of cytokines, which also includes TGF beta 1 and beta 2. These cytokines are secreted in precursor form consisting of a bioactive C-terminal domain attached to an N-terminal domain known as latency associated protein (LAP). Cleavage of LAP results in the mature protein, which functions as a disulfide-linked homodimer. As with all members of the family, TGF beta 3 is highly conserved across species, with mouse and human TGF beta 3 demonstrating 100% sequence homology and cross-species activity.The members of this family can be expressed by most cell types and exert pleiotropic effects, which include the suppression of B- and T-cell effector activity, mediation of tissue healing, and suppression of tumor proliferation. The promotion of CD4+CD25+ T-cell expansion is a newly discovered function of the TGF beta cytokines, and indicates an important role in the protection against autoimmunity.

Specifications

• Antigen: TGF beta-3
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 5% trehalose and No Preservative
• Gene Accession No.: P10600, P17125, Q07258
• Gene Symbols: TGFB3
• Immunogen: Sf 21-derived recombinant human TGF-beta 3 Ala301-Ser412 (Tyr340Phe).
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat, Multi-species
• Product Type: Antibody
• Isotype: IgG1

• Applications: Immunohistochemistry (Paraffin), Neutralization, Western Blot
• Clone: 20724
• Conjugate: Unconjugated
• Gene: TGFB3
• Gene Alias: ARVD; ARVD1; FLJ16571; H-TGF-b-3; LAP; Latency-associated peptide; LDS5; prepro-transforming growth factor beta-3; RNHF; TGF beta 3; Tgfb3; TGF-B3; Tgfb-3; TGF-beta3; TGF-beta-3; Transforming growth factor; transforming growth factor beta 3; transforming growth factor beta precursor; transforming growth factor beta-3; Transforming growth factor beta-3 proprotein; transforming growth factor, beta 3; transforming growth factor-beta3
• Host Species: Mouse
• Purification Method: Protein A/G
• Regulatory Status: RUO
• Gene ID (Entrez): 21809, 25717, 7043
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Lyophilized