TGF-beta RII, Goat anti-Mouse, APC, Polyclonal, R&D Systems™

Goat Polyclonal Antibody

Catalog Number	Mfr. No.	Quantity
FAB532A025	R&D Systems FAB532A025	25 Tests
FAB532A100	R&D Systems FAB532A100	100 Tests

Description

Most cell types express three sizes of receptors for TGF-beta. These are designated Type I (53 kDa), Type II (70-85 kDa), and Type III (250-350 kDa). The Type III receptor, a proteoglycan that exists in membrane-bound and soluble forms, binds TGF-beta 1, TGF-beta 2, and TGF-beta 3 but does not appear to be involved in signal transduction. The Type II receptor is a membrane-bound serine/threonine kinase that binds TGF-beta 1 and TGF-beta 3 with high affinity and TGF-beta 2 with a much lower affinity. The Type I receptor is also a membrane-bound serine/threonine kinase that apparently requires the presence of the Type II receptor to bind TGF-beta. Current evidence suggests that signal transduction requires the cytoplasmic domains of both the Type I and Type II receptors.The recombinant soluble TGF-beta Type II receptor is capable of binding TGF-beta 1, TGF-beta 3, and TGF-beta 5 with sufficient affinity to act as an inhibitor of these isoforms at high concentrations. The soluble receptor also binds TGF-beta 2, but with an affinity at least two orders of magnitude lower. Binding of TGF-beta 1, TGF-beta 3, and TGF-beta 5 to the soluble TGF-beta Type II receptor can also be demonstrated by using the soluble receptor as a capture agent on ELISA plates and this observation has been used as the basis for the development of immunoassays for these isoforms of TGF-beta.

Specifications

• Host Species: Goat
• Isotype: IgG
• Antigen: TGF-beta RII
• Format: Affinity Purified
• Regulatory Status: RUO
• Test Specificity: Detects mouse TGF-beta RII in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) TGF-beta RII is observed and less than 1% cross-reactivity with recombinant mouse TGF-beta RI and rhTGF-beta RIII is observed.
• Gene ID (Entrez): 7048
• Purification Method: Antigen Affinity-purified

• Conjugate: APC
• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse TGF-beta RII and S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse TGF-beta RII Ile24-Asp184 Accession # Q62312
• Monoclonal or Polyclonal: Polyclonal
• Primary or Secondary: Primary
• Applications: Flow Cytometry
• Gene Accession No.: Q62312
• Formulation: Supplied in a saline solution containing BSA and Sodium Azide. with Sodium Azide