Invitrogen™ THEM4 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA5143977

Catalog No. PIPA5143977

Description

Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL. Positive Control - WB: human HepG2 whole cell, human K562 whole cell. ICC/IF: PC-3 cell. Flow: U87 cell. Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

THEM4, also known as CTMP, binds specifically to the carboxy-terminal regulatory domain of PKB/Akt at the plasma membrane and acts as a negative regulator, reversing the phenotype of v-Akt-transformed cells. Hypermethylation of the THEM4 promoter and transcriptional downregulation of the gene has been reported in multiple glioblastomas, suggesting that epigenetic regulation of THEM4 may play a role in the progression of this cancer. Bioinformatic analysis, confirmed by in vitro testing, indicates that THEM4 is a broad-range, high activity acyl-CoA thioesterase. Recent reports have also indicated that TMEM4 is a mitochondrial protein whose overexpression is associated with an increase in mitochondrial membrane depolarization and caspase-3 and PARP cleavage, suggesting that THEM4 is involved in the apoptotic program.

Specifications

• Antigen: THEM4
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: THEM4
• Gene Alias: 2700077M13Rik; 4921507I02Rik; Acyl-CoA thioesterase THEM4; acyl-coenzyme A thioesterase THEM4; carboxyl-terminal modulator protein; C-terminal modulator protein; CTMP; RGD1304723; THEM4; Thioesterase superfamily member 4
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 117145
• Content And Storage: -20°C
• Form: Lyophilized

• Applications: ELISA, Flow Cytometry, Western Blot, Immunocytochemistry
• Concentration: 500 μg/mL
• Formulation: PBS with 4mg trehalose and no preservative
• Gene Accession No.: Q5T1C6
• Gene Symbols: THEM4
• Immunogen: E.coli-derived human THEM4 recombinant protein (Position: M1-T240).
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG