Invitrogen™ Themis Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA5143777

Catalog No. PIPA5143777

Description

The antibody detects a 72 kDa* protein corresponding to the molecular mass of themis on SDS-PAGE immunoblots of mouse spleen and human K562 cells.

Themis is a cytoplasmic protein that is essential for positive selection and the development of single-positive thymocytes. Highest levels of expression are found in CD4+CD8+ double-positive thymocytes, with lower expression found in mature T cells. In addition to Grb1, Themis has been found to associate with PLC gamma 1 and LAT and is quickly phosphorylated by SLP-76 following TCR signaling. There are 5 isoforms of mouse Themis that are produced as a result of alternative splicing.

Specifications

• Antigen: Themis
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Themis
• Gene Alias: bA325O24.3; bA325O24.4; C6orf190; C6orf207; E430004N04Rik; FLJ40584; Gasp; GRB2-associated protein; Grb2-associating protein; MGC163388; Protein THEMIS; Protein thylex; RGD1560849; signaling phosphoprotein specific for T cells; SPOT; THEMIS; thylex; thymocyte expressed molecule involved in selection; thymocyte selection associated; thymocyte selection pathway associated; thymocyte-expressed molecule involved in selection; Tsepa
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 210757, 387357, 498985
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: Western Blot
• Concentration: 1 mg/mL
• Formulation: PBS with 1mg/mL BSA, 50% glycerol and 0.05% sodium azide
• Gene Accession No.: Q8BGW0, Q8N1K5
• Gene Symbols: Themis
• Immunogen: Themis (N-terminal region) antibody was generated from a peptide that included amino acids in the N-terminal region of human themis. This sequence has high homology to the conserved region in rat and mouse themis.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG