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Thermo Scientific™ Luminaris HiGreen qPCR Master Mix
Description
Includes
Luminaris HiGreen qPCR Master Mix (2X) containing Hot Start Taq DNA Polymerase, UDG, dNTPs (with dUTP) and SYBR Green I in an optimized PCR buffer; Nuclease-Free Water
Ready-to-use qPCR master mixes with premixed UDG optimized for SYBR Green chemistry using standard cycling protocol. Master mixes available with or without blue color.
- UDG in the master mix to prevent carry-over contamination
- Equal performance of blue and colorless master mixes
- Versions with premixed ROX or fluorescein for different real-time platforms
- Standard cycling protocols
- Excellent qPCR performance
- Specificity: Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
- Sensitivity: single copy detection
- Wide linear range: accurate quantification across 9 orders of magnitude
- Reproducibility and convenience: ready-to-use 2X master mix
Specifications
Specifications
| Concentration | 2X |
| Content And Storage | Contains: • 4 x 12.5 mL Luminaris HiGreen qPCR Master Mix (2X) containing Hot Start Taq DNA Polymerase, UDG, dNTPs (with dUTP) and SYBR Green I in an optimized PCR buffer; sufficient for 5000 x 20 μL reactions • 2 x 30 mL Nuclease-Free Water Store at -20°C. |
| Detection Method | SYBR |
| GC-Rich PCR Performance | High |
| PCR Method | qPCR |
| Polymerase | Taq DNA Polymerase |
| Reaction Speed | Standard |
| For Use With (Equipment) | BioRad CFX96™, MJ Chromo4, MJ Opticon, Eppendorf MasterCycler RealPlex, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Cepheid SmartCycler, Corbett RotorGene |
| Product Line | Luminaris |
| Product Type | qPCR Master Mix |
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Frequently Asked Questions (FAQs)
The addition of yellow dye from the Yellow Sample Buffer to qPCR reaction has no influence on the efficiency, specificity, sensitivity or Cq values. Using the Yellow Sample Buffer is optional, but the dye helps to see in which wells the sample has been already added.
Luminaris Color HiGreen master mixes contain SYBR Green I double-stranded DNA binding dye.
Luminaris qPCR master mixes have all dTTP substituted with dUTP. dUTP presence does not influence the reaction speed and efficiency of the master mix. 100% substitution of dTTP with dUTP facilitates an efficient clearance of the contamination from previous PCR reactions.
Luminaris qPCR master mixes contain Hot Start Taq DNA Polymerase. This is a Taq DNA polymerase, which has been chemically modified by the addition of heat-labile groups to amino acid residues blocking enzyme activity. The Hot Start Taq DNA Polymerase has ultra low residual activity until thermally activated. Therefore reactions can be assembled at room temperature with no risk for the extension of non-specifically annealed primers or primer dimers and providing higher specificity for DNA amplification. The enzyme is activated by 10 minutes incubation at 95 degrees C.
When using a hot start polymerase it is not critical to start reactions immediately after setup. The Hot Start Taq DNA Polymerase in Luminaris qPCR Master Mixes has ultra low residual activity until thermally activated. Therefore reactions can be assembled and stored at room temperature for up to 72 hours with no risk for the extension of non-specifically annealed primers or primer dimers.
For Research Use Only. Not for use in diagnostic procedures.
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