Thermo Scientific™ MagJET NGS Cleanup and Size Selection Kit

Thermo Scientific MagJET NGS Cleanup and Size Selection kit is designed for efficient and robust DNA fragment library cleanup and size-selection from a variety of enzymatic reaction mixtures including PCR, ligation, adapter addition and DNA end-repair reaction mixes.

Supplier:  Thermo Scientific™ K2828

Catalog No. FERK2828

Description

Thermo Scientific MagJET NGS Cleanup and Size Selection kit is designed for efficient and robust DNA fragment library cleanup and size-selection from a variety of enzymatic reaction mixtures including PCR, ligation, adapter addition and DNA end-repair reaction mixes. The kit utilizes high-capacity MagJET paramagnetic bead technology that ensures efficient recovery of DNA fragment library within a desired fragment length range. The purified DNA fragments are free of any next-generation sequencing workflow inhibiting components, such as sequencing adapters, primer dimers, unincorporated nucleotides, enzymes or salts, and are ready-to-use in downstream NGS applications. No prior DNA fragment library purification is required before the cleanup and size-selection procedures.

The kit is suitable for cleanup or size selection of 5 ng to 5 μg of DNA fragment library. MagJET cleanup and adapter removal protocols can easily be automated for higher throughput on automatic platforms such as Thermo Scientific KingFisher Flex and Thermo Scientific KingFisher Duo.

Highlights
• Robust—reliable cleanup and size selection from different reaction mixtures and buffers
• Flexible—user-defined size-selection of exact DNA fragment range
• NGS-grade purity—complete removal of NGS adapters, primers, nucleotides, enzymes and other reaction components inhibiting NGS workflow
• High throughput compatible—easy to automate on the Thermo Scientific KingFisher magnetic particle processors for high-throughput sample handling

Applications
DNA fragment library cleanup and size-selection for downstream use in next generation sequencing workflows.

Includes
• MagJET Magnetic Beads
• Binding Solution
• Wash Buffer (concentrated)
• Elution Buffer
• DNA Fragment Mix

Specifications

• Content And Storage: • 1 mL DNA Fragment Mix; -20°C
  • 110 μL MagJET Magnetic Beads; 4°C
  • 8 mL Binding Buffer (concentrated); room temperature
  • 3 mL Wash Buffer (concentrated); room temperature
  • 2 x 1.5 mL Elution Buffer; room temperature
  
  
• Sample Type: Enzymatic Reactions (PCR, ligation, adapter addition, DNA end-repair reaction)
• Final Product Type: DNA
• For Use With (Application): PCR, Southern blotting, sequencing, nucleic acid labeling, hybridization
• Quantity: 10 Preps
• Yield: Up to 700 bp

• Isolation Technology: Magnetic Bead
• Elution Volume: 10 to 50 μL
• For Use With (Equipment): KingFisher™ Flex, KingFisher™ Duo Prime
• High-throughput Compatibility: High-throughput Compatible
• Shipping Condition: Room Temperature
• Starting Material Amount: 100 μL (5 ng to 5 μg DNA fragments)

For Research Use Only. Not for use in diagnostic procedures.