Thermo Scientific Pierce Cypridina Luciferase Flash Assay Kit
Assay regulatory element transcriptional activity in mammalian cell culture media and whole cell lysate with this highly sensitive flash-based luciferase kit.
Manufacturer: Thermo Scientific 16168
The Thermo Scientific Pierce Cypridina Luciferase Flash Assay Kit provides researchers with a highly sensitive assay for transcriptional activity of regulatory elements in mammalian cell culture media and whole cell lysate.
This Flash Assay Kit contains reagents for measuring the activity of Cypridina luciferase in mammalian cell culture media and lysates. When used with Thermo Scientific Cypridina Luc Vectors, the kit provides an extremely sensitive bioluminescent reporter assay system for secreted or intracellular detection of promoter or pathway activity. Cypridina luciferase is highly secreted into the cell culture media, allowing for live cell monitoring of reporter activity. The signal produced by Cypridina is considerably greater than signal from either firefly or Renilla luciferases assayed under similar conditions.
- Sensitive high sensitivity allows utilization of smaller numbers of cells
- Cost effective sensitive assays result in decreased reagent consumption
- Secreted luciferase allows real-time assays and kinetic studies without destroying cells
- Ideal for co-transfection uses a different substrate than Renilla, Gaussia, or firefly luciferases
- Compatible assay reagents compatible with other Cypridina or Vargula luciferases
- Time-saving assays using secreted luciferases require minimal sample handling
- Automation-friendly amenable to high throughput screens
- Convenient contains a universal cell lysis buffer and optimized flash assay reagent
- Safe allows one to perform non-radioactive assays
Cell lysis buffer, reaction buffer and substrate
Cypridina luciferase and luminometer or other instrument capable of monitoring luminescence, such as Thermo Scientific Luminoskan Ascent and Varioskan Flash Microplate Readers.
Promoter studies for analyzing cis- regulatory elements and trans-acting factors; Drug screening; siRNA and miRNA screening; Multiplexed assays to study off-target effects; Secretory pathway / protein localization reporter assays; Signal transduction pathway analysis; RNA splicing studies
|Reporter Enzyme, Luciferase Reporter Assay|
|• Cypridina Flash Assay Buffer, 5 mL, store at 4°C
• Vargulin (1X), 5 µL, store at -20°C
• 2X Cell Lysis Buffer, 6 mL, store at room temperature
Upon receipt store kit at -20°C or store individual components as indicated above.
|Vargulin (Cypridina Luciferin)|
|384-well plate, 96-well plate|
|Luciferase, Cypridina Luciferase|
|100 rxn kit|
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