Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

Thermo Scientific™ Pierce™ Firefly Luc One-Step Glow Assay Kit

Perform firefly luciferase assays in high-throughput screening applications.

$157.50 - $763.50

Products 2
Catalog Number Mfr. No. Quantity Sufficient For Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Sufficient For Price Quantity & Availability  
View Documents Promotion Details
Thermo Scientific™
1000 Reaction Kit 1000 luciferase assays in microplate wells
Each for $763.50
Add to cart
View Documents Promotion Details
Thermo Scientific™
100 Reaction Kit 100 luciferase assays in microplate wells
Each for $157.50
Add to cart


The Thermo Scientific Pierce Firefly One-Step Luc Glow Assay Kit measures firefly luciferase activity in mammalian cell culture using a single reagent-addition step, making it ideal for high-throughput screening (HTS) applications.

This glow-type luciferase assay kit is designed for use with cultured mammalian cells that are transfected with vectors expressing the appropriate firefly luciferase reporter enzyme. Several features make the kit especially suitable for luminometers without injectors and for high-throughput screening (HTS) applications. The convenient, one-step, homogenous protocol minimizes handling steps to support the use of automation. Simply combine the supplied substrate solution and assay buffer to make a single working solution, and then add it to microplate wells containing the transfected, treated cells. The stable signal output (glow) of this system provides flexibility with regard to incubation time. When used with Thermo Scientific Firefly Luc Plasmids, the kit provides a highly sensitive bioluminescent reporter assay system for the detection of promoter or pathway activity.


  • Sensitive - highly sensitive detection of firefly luciferase activity
  • Convenient - one-step, mix-and-read protocol; no additional reagents required
  • Stable - increased signal half-life and stability compared to flash assays
  • Simple - does not require a luminometer with injectors
  • Automation-friendly - amenable to high throughput assays
Assay buffer and substrate solution (100X)
Firefly luciferase and luminometer or other instrument capable of monitoring luminescence, such as Thermo Scientific Luminoskan Ascent and Varioskan Flash Microplate Readers.
  • Promoter studies for analyzing cis- and trans-acting regulatory elements
  • Drug screening
  • siRNA and miRNA screening
  • Multiplexed assays to study off-target effects
  • Protein localization reporter assays
  • Signal transduction pathway analysis
Product Details:
Firefly luciferase is a 62kDa protein produced in nature by several species of the Lampyridae family of beetles, which includes the genera Photinus and Luciola. Thermo Scientific Red Firefly Luciferase has an emission range of 560-700nm (λmax = 613nm). Bioluminescent signal from firefly luciferase originates from the oxidation of D-Luciferin. Light output captured using a luminometer can be correlated with the amount of firefly luciferase protein produced and used to determine the activity of the promoter driving firefly luciferase expression. Firefly luciferase bioluminescence provides researchers with a non-radioactive assay for measuring transcriptional activity of regulatory elements. Firefly luciferase reporter activity can be used to determine promoter activity, to study upstream pathway activity in the cell, or to determine effect of activators or inhibitors.

Safety and Handling

Safety and Handling

Product Certifications
Provide Content Correction

We continue to work to improve your shopping experience and your feedback regarding this content is very important to us. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Cancel Submit