Human Neutrophil elastase, Native Protein, Invitrogen™

Description

Native Protein

Neutrophil Elastase (human) Native Protein, RP-77526, from Invitrogen.

Leukocyte elastase is one of several hydrolytic enzymes contained in the azurophil granules of human neutrophils exhibiting a relative molecular weight of 29.5 kDa. The enzyme differs from pancreatic elastase in specificity on synthetic substates and in inhibitor sensitivity. Leukozyte elastase shows only moderate sequence homology with pancreatic elastase. It has a very narrow specificity, cleaving Val-X bonds preferentially, and Ala-X links to a lesser extent. It has been shown to degrade elastin, cartilage proteoglycans, several collagens and fibronectin. Physiologically, it is involved in the degradation of foreign materials ingested during phagocytosis. Damage to connective tissue caused by leakage of enzymes is normally limited by proteinase inhibitors. It has been suggested that insufficiant levels of these inhibitors leads to pulmonary emphysema, and elastase in particular has been implicated in abnormal lung connective tissue turnover. RP-77526 is native Neutrophil Elastase protein purified from whole human blood samples. RP-77526 may be used as a control with corresponding anti-Neutrophil Elastase antibodies. RP-77526 is prepared from whole human blood shown to be non-reactive for HbsAG, anti HCV, anti-HBc, and negative for anti-HIV1 2 by FDA approved tests.

Specifications

• Accession Number: P08246
• Concentration: 0.2mg/mL
• For Use With (Application): Control
• Formulation: 0.01M acetic acid with no preservative; pH 5.5
• Gene ID (Entrez): 1991
• Name: Human Neutrophil elastase
• pH Range: 5.5
• Quantity: 25 μg
• Source: Human
• Storage Requirements: -80°C, Avoid Freeze/Thaw Cycles
• Regulatory Status: RUO
• Common Name: Human Neutrophil elastase
• Gene Symbol: ELANE
• Specific Reactivity: Human
• Conjugate: Unconjugated
• Cross Reactivity: Hu
• Species: Human
• Recombinant: Native
• Form: Liquid
• Protein Subtype: Native
• Purity or Quality Grade: purified

in vitro research only