Thermo Scientific™ UltraLink™ Iodoacetyl Micro Peptide Coupling Kit, 0.1 mL

Description

Covalently immobilize cysteine-peptides (sulfhydryl-containing molecules) for antibody affinity purification with these haloacetyl-activated polyacrylamide beads.

Thermo Scientific™ UltraLink Iodoacetyl Resin and Peptide Coupling Kit enable the covalent immobilization of cysteine-containing peptides for use in antibody purification and other affinity protocols.

The beaded UltraLink Iodoacetyl Resin is a hydrophilic copolymer of polyacrylamide and azlactone having a rigid polymeric structure with high surface area and pore volume. Each azlactone group has been modified to form a 15-atom spacer arm that terminates in an iodoacetyl group, which is capable of reacting with sulfhydryl groups (e.g., side chain of reduced cysteine residues) to covalently immobilize peptide or other sulfhydryl-containing ligands. The bead structure and efficiently coupling chemistry of Iodoacetyl-Activated UltraLink Biosupport results in high protein binding capacity, high linear flow rates, low nonspecific binding and overall superior performance in affinity chromatography. UltraLink Resins are ideal for medium pressure applications such as FPLC.

The Micro Peptide Coupling Kit is a microcentrifuge spin column kit for immobilizing small amounts (25 to 250μg) of sulfhydryl-containing peptides (e.g., cysteine-terminated peptides) onto the resin to create a small, reusable affinity column. The coupling and affinity purification procedures are optimized for small sample volumes (200 to 300μL). Wash and elution steps are achieved rapidly and efficiently with the convenient microcentrifuge spin columns. Each kit contains sufficient reagents for 10 coupling reactions and 20 affinity purifications. The kit is ideal for immobilizing peptide antigens that contain a terminal cysteine residue for use in purifying specific antibodies from small serum, ascites or culture supernatant samples.

Highlights:

• High coupling efficiency and capacity – immobilizes sulfhydryl- containing proteins or other ligands with high efficiency and coupling capacity in one hour

• Specific and leak-proof coupling chemistry – reacts specifically with sulfhydryl groups (reduced thiols), resulting in thioether bonds that are stable for use in many affinity purification procedures

• Simple coupling conditions – perform immobilization reaction in any of a variety of buffers; coupling is most efficient and specific at pH 8.0 to 8.5; coupling compatible with or without organic solvent

• Excellent reusability – prepared affinity resin can be used with typical binding and elution procedures for many cycles of affnity purification without significant loss of binding capacity

• Durable, high-performance resin – porous beads have a 60μm diameter, can withstand 100 psi (6.9 bar) and allow for linear flow-through rates of 3000cm/hour

Micro Peptide Coupling Kit (Part No. 20485):

• Optimized for small scale – ideal for coupling small amounts (25 to 250μg) of sulfhydryl-containing peptide or protein for purification of specific antibodies from crude serum

• High-performance affinity resin – uses durable, polyacrylamide-based UltraLink Iodoacetyl Resin for specific reaction to sulfhydryl groups

• Efficient coupling chemistry – immobilization efficiency >85% (as measured with 1 hour reaction using insulin, calcitonin and osteocalcin peptides)

• Fast and easy to use – perform wash and elution steps using a microcentrifuge

• Reusable – use prepared peptide resin several times with no significant loss of capacity

Includes:

Micro Peptide Coupling Kit contains microcentrifuge spin columns containing UltraLink Iodoacetyl Resin, coupling buffer, L-cysteine (for quenching), wash solution, PBS, elution buffer and collection tubes

Specifications

• Capacity (Metric): 25 to 250 μg of Peptide or Protein per Reaction
• Description: UltraLink Iodoacetyl Micro Peptide Coupling Kit
• Content And Storage: Sufficient For: Immobilizing 25–250 μg of peptide or protein per microcentrifuge column; Includes buffers for 20 affinity purifications
  • UltraLink Iodoacetyl Spin Columns, 0.1 mL, 10 columns
  • Coupling Buffer, 100 mL
  • L-Cysteine·HCl, 100 mg
  • Wash Solution, 25 mL
  • PBS Pack (makes 500 mL), 1 pack
  • IgG Elution Buffer, 50 mL
  • Collection Tubes, 200 tubes
  Store at 4°C protected from light.
• Product Line: UltraLink™
• Quantity: 1 Kit, 10 Columns
• Type: Peptide Coupling Kit
• Stationary Phase: Haloacetyl
• Sufficient For: 10 Reactions
• Format: Kit, Microcentrifuge, Spin Column
• Column Type: Affinity, UltraLink™ Resin