Cytiva Thermo Sequenase DNA Polymerase

Description

Novel thermostable DNA polymerase that uses dideoxynucleotide triphosphates (ddNTPs) as readily as deoxynucleotide (dNTP) substrates

• Uniform and easy-to-read band patterns
• Better incorporation of ddNTPs helps avoid secondary structures that can cause unreadable sequences
• Read-through of difficult sequences (e.g., GC-rich), due to the ability of thermostable enzymes to function at elevated temperature used for sequencing
• ddNTPs are chain-elongating inhibitors of DNA polymerase, which are used in the Sanger method for DNA sequencing
• Each nucleotide base of this particular type has a probability of being bonded not to a deoxynucleotide but rather a dideoxynucleotide, which ends chain elongation
• An excellent choice for cycle sequencing where template quantity limitations preclude the use of T7 Sequenase DNA polymerase
• Can also be used in many SNP genotyping, genotyping by sequencing, or genome sequencing applications where primer extension is employed

Specifications

• Content And Storage: -20°C, Solution in 20 mM Tris-HCl, pH 8.5, 0.1 mM EDTA, 0.5% Tween 20 (v/v), 0.5% Nonidet P-40 (v/v), 1 mM DTT, 100 mM KCl, 50% glycerol
• Description: Thermo Sequenase DNA Polymerase
• Quantity: 10,000 Units
• For Use With (Application): Sequencing, Genotyping
• No. of Reactions: 32 units/μL
• Includes: Thermo Sequenase/TAP, Dilution Buffer, and Reaction Buffer
• Source: E. coli clones that overproduce the protein