Cytiva Thermo Sequenase DNA Polymerase

Novel thermostable DNA polymerase that uses dideoxynucleotide triphosphates (ddNTPs) as readily as deoxynucleotide (dNTP) substrates

Manufacturer:  Cytiva E79000Z

Catalog No. 09-928-127

Description

• Uniform and easy-to-read band patterns
• Better incorporation of ddNTPs helps avoid secondary structures that can cause unreadable sequences
• Read-through of difficult sequences (e.g., GC-rich), due to the ability of thermostable enzymes to function at elevated temperature used for sequencing
• ddNTPs are chain-elongating inhibitors of DNA polymerase, which are used in the Sanger method for DNA sequencing
• Each nucleotide base of this particular type has a probability of being bonded not to a deoxynucleotide but rather a dideoxynucleotide, which ends chain elongation
• An excellent choice for cycle sequencing where template quantity limitations preclude the use of T7 Sequenase DNA polymerase
• Can also be used in many SNP genotyping, genotyping by sequencing, or genome sequencing applications where primer extension is employed

Specifications

• Storage Requirements: -20°C, Solution in 20 mM Tris-HCl, pH 8.5, 0.1 mM EDTA, 0.5% Tween 20 (v/v), 0.5% Nonidet P-40 (v/v), 1 mM DTT, 100 mM KCl, 50% glycerol
• For Use With (Application): DNA Sequencing, and SNP Genotyping Applications
• Quantity: 10,000 Units
• Product Name: Thermo Sequenase DNA Polymerase

• No. of Reactions: 32 units/µL
• Source: E. coli clones that overproduce the protein
• Includes: Thermo Sequenase/TAP, Dilution Buffer, and Reaction Buffer
• Item Description: One unit of enzyme incorporates 10 nmol of dNTPs into acid-insoluble material in 30 min (at 74°C)