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Cytiva Thermo Sequenase DNA Polymerase

Novel thermostable DNA polymerase that uses dideoxynucleotide triphosphates (ddNTPs) as readily as deoxynucleotide (dNTP) substrates

Manufacturer:  Cytiva E79000Z

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Catalog No. 09-928-127


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Description

Description

  • Uniform and easy-to-read band patterns
  • Better incorporation of ddNTPs helps avoid secondary structures that can cause unreadable sequences
  • Read-through of difficult sequences (e.g., GC-rich), due to the ability of thermostable enzymes to function at elevated temperature used for sequencing
  • ddNTPs are chain-elongating inhibitors of DNA polymerase, which are used in the Sanger method for DNA sequencing
  • Each nucleotide base of this particular type has a probability of being bonded not to a deoxynucleotide but rather a dideoxynucleotide, which ends chain elongation
  • An excellent choice for cycle sequencing where template quantity limitations preclude the use of T7 Sequenase DNA polymerase
  • Can also be used in many SNP genotyping, genotyping by sequencing, or genome sequencing applications where primer extension is employed
Specifications

Specifications

-20°C, Solution in 20 mM Tris-HCl, pH 8.5, 0.1 mM EDTA, 0.5% Tween 20 (v/v), 0.5% Nonidet P-40 (v/v), 1 mM DTT, 100 mM KCl, 50% glycerol
DNA Sequencing, and SNP Genotyping Applications
10,000 Units
Thermo Sequenase DNA Polymerase
32 units/µL
E. coli clones that overproduce the protein
Thermo Sequenase/TAP, Dilution Buffer, and Reaction Buffer
One unit of enzyme incorporates 10 nmol of dNTPs into acid-insoluble material in 30 min (at 74°C)
SDS
Documents
Product Certifications
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