Invitrogen™ Thioredoxin 1 Polyclonal Antibody

Catalog No.	Quantity
PIPA595589	100 μg

Catalog No. PIPA595589 Supplier Invitrogen™ Supplier No. PA595589

Description

Rabbit Polyclonal Antibody

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 μg/mL. Positive Control - WB: rat kidney tissue, rat small intestine tissue, rat spleen tissue, rat lung tissue, rat C6 whole cell, mouse kidney tissue, mouse small intestine tissue, mouse lung tissue, mouse heart tissue, mouse NIH/3T3 whole cell. IHC: mouse small intestine tissue, rat spleen tissue. ICC/IF: NIH3T3 cell, LLC Cell. Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Thioredoxins (Trx) are small, multi-functional proteins with oxidoreductase activity and are ubiquitous in essentially all living cells. Trx contains a redox-active disulfide/dithiol group within the conserved Cys-Gly-Pro-Cys active site. The two cysteine residues in the conserved active centers can be oxidized to form intramolecular disulfide bonds. Reduction of the active site disulfide in oxidized Trx is catalyzed by Trx reductase with NADPH as the electron donor. The reduced Trx is a hydrogen donor for ribonucleotide reductase, the essential enzyme for DNA synthesis, and a potent general protein disulfide reductase with numerous functions in growth and redox regulations. Specific protein disulfide targets for reduction by Trx include protein disulfide-isomerase (PDI) and a number of transcription factors such as p53, NF-kB and AP-1 (T1-151). Trx is also capable of removing H2O2, particularly when it is coupled with either methionine sulfoxide reductase or several isoforms of peroxiredoxins.

Specifications

• Antigen: Thioredoxin 1
• Applications: ELISA, Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Classification: Polyclonal
• Concentration: 500 μg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 4mg trehalose and 0.05mg sodium azide
• Gene: Txn1
• Gene Accession No.: P10639, P11232
• Gene Alias: ADF; ATL-derived factor; AW550880; DKFZp686B1993; MGC61975; RP11-427L11.1; SASP; surface-associated sulphydryl protein; testicular tissue protein Li 199; thioredoxin; thioredoxin 1; thioredoxin delta 3; TRDX; TRX; TRX1; Txn; TXN delta 3; Txn1
• Gene Symbols: Txn1
• Host Species: Rabbit
• Immunogen: E.coli-derived mouse Thioredoxin TRX recombinant protein (Position: V2-A105).
• Purification Method: Affinity chromatography
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 116484, 22166
• Target Species: Mouse, Rat
• Content And Storage: -20°C
• Product Type: Antibody
• Form: Lyophilized
• Isotype: IgG