Thrombospondin-1 Mouse anti-Human, Rat, Clone: 133, MilliporeSigma™

Mouse Monoclonal Antibody

Supplier:  MilliporeSigma MABT879

Catalog No. MABT879

Description

Detect Thrombospondin-1 using this Anti-Thrombospondin-1 Antibody, clone 133 validated for use in Western Blotting, Immunohistochemistry (Paraffin), Neutralizing, ELISA.

Specifications

• Antigen: Thrombospondin-1
• Classification: Monoclonal
• Concentration: Please refer to lot specific datasheet.
• Gene Symbols: THBS,THBS1,TSP,TSP1
• Immunogen: Alkaline-stripped human platelet thrombospondin-1 (sTSP-1).
• Quantity: 100 μg
• Research Discipline: Cell Structure
• Gene ID (Entrez): NP_003237
• Target Species: Human, Rat
• Form: Purified

• Applications: Western Blot, Immunohistochemistry (Paraffin), Neutralization, ELISA
• Clone: 133
• Conjugate: Unconjugated
• Host Species: Mouse
• Purification Method: Protein G Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Clone 133 reacted with human thrombospondin-1 (hTSP-1), but not hTSP-2, mouse TSP-1 (mTSP-1), or mTSP-2. Clone 133 detected the C-terminal 50 kDa chymotryptic fragment of stripped TSP as well as recominant fragements containing the Calcium-binding type-3 repeats (a.a. 692-945) region (Annis, D.S., et al. (2006). J. Thromb. Haemost. 4(2):459-468; Schultz-Cherry, S., and Murphy-Ullrich J.E. (1993). J. Cell Biol. 122(4):923-932).
• Content And Storage: Stable for 1 year at -20°C from date of receipt.Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
• Isotype: IgG2b κ