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TIGIT Mouse anti-Human, PerCP, Clone: 741182, R&D Systems™

Mouse Monoclonal Antibody

Manufacturer:  R&D Systems FAB7898C025

Catalog No. FAB7898C025


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Description

Description

TIGIT (T cell Immunoreceptor with Ig and ITIM domains), also called Vstm3 (V-set and transmembrane domain-containing 3), Vsig9 (V-set and Ig domain-containing 9) and WUCAM (Washington University cell adhesion molecule) is a 30-34kDa type I transmembrane protein that is a member of the CD28 family within the Ig superfamily of proteins (1-4). Human TIGIT cDNA encodes 244 amino acids (aa) including a 21 aa signal sequence, a 120 aa extracellular region with a V-type Ig-like domain and two potential N-glycosylation site, a 21 aa transmembrane sequence, and an 82 aa cytoplasmic domain with an ITIM motif (5). A 170 aa variant diverges after aa 166 (5). Within the ECD, human TIGIT shares only 68-75% aa sequence identity with mouse, porcine, canine, equine and bovine TIGIT. TIGIT is expressed on NK cells and subsets of activated, memory and regulatory T cells, and particularly on follicular helper T cells within secondary lymphoid organs (1, 2, 6-8). It binds to CD155/PVR/Necl-5 and Nectin-2/CD112/PVRL2 that appear on dendritic cells (DC) and endothelium (1-3, 7). Binding of TIGIT by DC induces IL-10 release and inhibits IL-12 production (2). Ligation of TIGIT on T cells down-regulates TCR-mediated activation and subsequent proliferation, while NK cell TIGIT ligation blocks NK cell cytotoxicity (6-8). Through CD155 and Nectin-2, which also interact with DNAM-1/CD226 and CD96/Tactile, TIGIT is part of an interacting network of Ig superfamily members that may augment or oppose each other (3, 4, 6, 7). In particular, TIGIT binding to CD155 can antagonize the effects of DNAM-1 (6, 7). Soluble TIGIT is able to compete with DNAM-1 for CD155 binding and attenuates T cell responses, while mice lacking TIGIT show increased T cell responses and susceptibility to autoimmune challenges (2, 3, 8).
Specifications

Specifications

TIGIT
741182
Flow Cytometry 10 uL/10^6 cells
Q495A1
TIGIT
Mouse myeloma cell line NS0-derived recombinant human TIGIT Met1-Pro141 Accession # Q495A1
Protein A or G purified from hybridoma culture supernatant
RUO
Primary
201633
Human
Flow Cytometry
PerCP
Supplied in a saline solution containing BSA and Sodium Azide. with Sodium Azide
Purified
Mouse
IgG2b
25 Tests
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied.
Monoclonal
Detects human TIGIT in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse TIGIT is observed.
SDS
Documents
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