MilliporeSigma™ TIM-1, Rat, Unlabeled, Clone: 3B3,

Catalog No.	Quantity	Conjugate
MABF245	100 μg	Unconjugated

Catalog No. MABF245 Supplier MilliporeSigma™ Supplier No. MABF245

Description

Rat Monoclonal Antibody

Hepatitis A virus cellular receptor 1 homolog (UniProt Q5QNS5; also known as HAVcr-1, Kidney injury molecule 1, KIM-1, T cell immunoglobulin and mucin domain-containing protein 1, T-cell immunoglobulin mucin receptor 1, T cell membrane protein 1, TIM-1, TIMD-1) is encoded by the Havcr1 (also known as Kim1, Tim1, Timd1) gene (Gene ID 171283) in murine species. TIM‐1/KIM‐1 is a phosphatidylserine phagocytosis and scavenger receptor expressed on kidney proximal tubule epithelial cells and, at a much lower level, also among a subset of immune cells. Upon kidney injury, TIM‐1 is upregulated more than a thousand fold and assists tissue injury recovery through phagocytosis of apoptotic cells and cellular debris. KIM‐1‐mediated phagocytosis leads to pro‐tolerogenic antigen presentation, which suppresses CD4+ T‐cell proliferation and increases the percentage of regulatory T-cells in an autophagy gene‐dependent manner. Phagocytosis mediated by KIM‐1 in epithelial cells therefore limits inflammation by both removing dead cells and inducing anti‐inflammatory Treg signaling. Human HAVCR1 polymorphisms are linked to various immunological disorders including allergy, asthma, rheumatoid arthritis, atopic dermatitis, and lupus. TIM-1 is produced with a signal peptide (a.a. 1-21) sequence which is removed posttranslationally to yield the mature protein with a large extracellular region (a.a. 21-290 in human and 22-237 in mouse) composed of a V-type Ig-like domain (a.a. 21-121 in human and 22-122 in mouse) and a mucin domain (the rest of the extracellular region), followed by a transmembrane domain (a.a. 238-258), and a cytoplasmic tail (a.a. 312-359 in human and 259-305 in mouse) with tyrosine phosphorylation sites.

Specifications

• Antigen: TIM-1
• Applications: Functional Assay
• Classification: Monoclonal
• Clone: 3B3
• Concentration: Please refer to lot specific datasheet.
• Conjugate: Unconjugated
• Formulation: Purified rat IgG2a in buffer containing PBS without preservatives.
• Gene: Havcr1, Kim1, Tim1, Timd1
• Gene Accession No.: Q5QNS5
• Host Species: Rat
• Immunogen: Recombinant TIM-1 Ig fusion consists of mouse TIM-1 signal and IgV domains linked to the hinge-CH2-CH3 domains of mouse IgG2a (Umetsu, S.E., et al. (2005). Nat. Immunol.6(5):447-454).
• Purification Method: Protein G Purified
• Quantity: 100 μg
• Regulatory Status: RUO
• Research Discipline: Inflammation & Immunology
• Primary or Secondary: Primary
• Gene ID (Entrez): NP_001160103
• Test Specificity: Clone 3B3 detected recombinant murine TIM-1, but not TIM-3 or TIM-4, Ig fusion. Clone 3B3 immunostained the surface of TIM-1-transfected, but not untransfected 300.19 mouse pre-B-cells (Umetsu, S.E., et al. (2005). Nat. Immunol.6(5):447-454). Clone 3B3 and clone RMT1-10 (Mfr. No. MABF225) exhibited opposite effects toward T cell antigen responses (Xiao, S., et al. (2007). J. Exp. Med. 204(7):1691-1702).
• Target Species: Mouse
• Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
• Form: Purified
• Isotype: IgG2a κ

For Research Use Only. Not for use in diagnostic procedures.