Novus Biologicals™ TIM-3 Antibody Pair [HRP]

Description

Monoclonal Antibody Pair

This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human TIM-3.

Specifications

• Assay Range: 31.25 pg/mL
• Assay Sensitivity: 31.25-2000 pg/mL
• Assay: Sandwich ELISA
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: Antibody Pairs
• Specificity: This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human TIM-3.
• Target Species: Human
• For Use With (Application): Sandwich ELISA
• Gene Alias: CD366, FLJ14428, HAVcr-2, hepatitis A virus cellular receptor 2, kidney injury molecule-3, T cell immunoglobulin mucin 3, T cell immunoglobulin mucin-3, T-cell immunoglobulin and mucin domain-containing protein 3, TIM 3, Tim-3, TIM3 T-cell membrane protein 3, TIMD-3, TIMD3KIM-3
• Gene ID (Entrez): 84868
• Gene Symbol: HAVCR2
• Host Species: Rabbit/Mouse
• Kit Contents:
  Standard: Each vial contains 85 ng of recombinant Human TIM-3.
  Capture Antibody: 1 mg/mL of rabbit anti-Human TIM-3 monoclonal antibody (in PBS, pH 7.4).
  Detection Antibody: 0.2 mg/mL of mouse anti-Human TIM-3 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4C).
• Quantity: 15 Plates
• Regulatory Status: RUO
• Research Discipline: Immunology, Innate Immunity
• Storage Requirements: Storage is content dependent.
• Target: TIM-3
• Technique: 1. Capture Antibody: Dilute to a working concentration of 2 μg/mL in PBS before coating. 2. Detection Antibody: Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. 3. Standard: Reconstitute with 1 ml detection antibody dilution buffer. After reconstitution, store at -20 to -80°C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 2000 pg/mL is recommended.