TIM-4 Monoclonal Antibody (54 (RMT4-54)), PerCP-eFluor™ 710, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
46-586-680	Invitrogen™ 46586680	25 μg
50-112-3846	Invitrogen™ 46586682	100 μg

Description

Description: The monoclonal antibody 54 (RMT4-54) recognizes mouse Tim-4 (T cell, Ig, mucin). Unlike other Tim family members, Tim-4 is not expressed on T lymphocytes but rather found on antigen presenting cells such as lymphoid and myeloid DC's and macrophages. Tim-4 has been shown to interact with Tim-1 thereby suggesting a role of Tim-4 in the balance of Th1/Th2 regulation. Additionally Tim-4 has been shown to be a receptor for phosphatidylserine and may play a role in engulfment of apoptotic cells. Expression is found on a subset of CD11c+ splenic cells. Applications Reported: This 54 (RMT4-54) antibody has been reported for use in flow cytometric analysis. Applications Tested: This 54 (RMT4-54) antibody has been tested by flow cytometric analysis of mouse resident peritoneal exudate cells. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. PerCP-eFluor™ 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative.

TIM-4 Monoclonal antibody specifically detects TIM-4 in Mouse samples. It is validated for Flow Cytometry.

Specifications

• Antigen: TIM-4
• Concentration: 0.2 mg/mL
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Alias: B430010N18Rik; RGD1564516; smuckler; soluble TIM 4; Spleen, mucin-containing, knockout of lymphotoxin protein; sTIM 4; T cell immunoglobulin and mucin domain containing 4; T-cell immunoglobulin and mucin domain containing 4; T-cell immunoglobulin and mucin domain-containing protein 4; T-cell immunoglobulin mucin receptor 4; T-cell membrane protein 4; Tim4; TIM-4; TIMD4; TIMD-4
• Gene Symbols: TIMD4
• Primary or Secondary: Primary
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Gene: TIMD4

• Clone: 54 (RMT4-54)
• Applications: Flow Cytometry
• Conjugate: PerCP-eFluor 710
• Host Species: Rat
• Target Species: Mouse
• Gene Accession No.: Q6U7R4
• Gene ID (Entrez): 276891
• Isotype: IgG2a κ
• Purification Method: Affinity Chromatography
• Product Type: Antibody

For Research Use Only.