Invitrogen™ TIN2 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA578469

Catalog No. PIPA578469

Description

Positive Control: IMR32 whole cell extract, 30 μg IMR32 nuclear extract Predicted Reactivity: Chimpanzee (99%), Bovine (80%) Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

TRF1 is a negative regulator of telomere length maintenance. Recently two proteins, tankyrase and Tin2, which bind to mammalian TRF1, have been identified. Tankyrase is a protein with homology to ankyrins as well as to the catalytic domain of poly (adenosine diphosphate-ribose) polymerase (PARP). Tankyrase localizes to telomeres by binding to the telomeric repeat binding factor 1 (TRF1) through its ankyrin repeats. Tankyrase exhibits PARP activity functioning as acceptors for adenosine diphosphate (ADP)-ribosylation. Since ADP-ribosylation of TRF1 diminishes its ability to bind to telomeric DNA, this suggests that telomere function in human cells is regulated by poly (ADP)-ribosylation. Both the cell cycle and TRF1 may regulate the subcellular localization of tankyrase. The cDNA coding for TIN2 protein was isolated as interacting partner of TRF1 from a yeast two-hybrid cDNA library screening. Tin2 localizes to telomeres and is essential for proper regulation of telomere length. TIN2, like TRF1, is widely and constitutively expressed, suggesting that these proteins act together to counterbalance telomere elongation by telomerase. However, endogenous TIN2, like endogenousTRF1, is not highly expressed, and therefore may be difficult to visualize by immunostaining in certain cell systems.

Specifications

• Antigen: TIN2
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Tinf2
• Gene Alias: AW552114; D14Wsu146e; DKCA3; TERF1 (TRF1)-interacting nuclear factor 2; TERF1 interacting nuclear factor 2; TERF1-interacting nuclear factor 2; tin; TIN2; Tin-2; TINF2; TRF1-interacting nuclear factor 2; TRF1-interacting nuclear protein 2
• Host Species: Rabbit
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 26277
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Western Blot
• Concentration: 1 mg/mL
• Formulation: PBS with 1% BSA, 20% glycerol and 0.01% thimerosal; pH 7
• Gene Accession No.: Q9BSI4
• Gene Symbols: Tinf2
• Immunogen: Full length human TIN2 Recombinant protein.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

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