Invitrogen™ TIN2 Recombinant Rabbit Monoclonal Antibody (4W5P2)

Catalog No.	Quantity
PIMA538210	100 μL

Catalog No. PIMA538210

Supplier: Invitrogen™ MA538210

Description

Rabbit Recombinant Monoclonal Antibody

Positive Samples: HepG2, A-549, Mouse testis, Mouse thymus, Rat lung, Rat testis Immunogen sequence: NLAEPMEQNP PQQQRLALHN PLPKAKPGTH LPQGPSSRTH PEPLAGRHFN LAPLGRRRVQ SQWASTRGGH KERPTVMLFP FRNLGSPTQV ISKPESKEEH.

TRF1 is a negative regulator of telomere length maintenance. Recently two proteins, tankyrase and Tin2, which bind to mammalian TRF1, have been identified. Tankyrase is a protein with homology to ankyrins as well as to the catalytic domain of poly (adenosine diphosphate-ribose) polymerase (PARP). Tankyrase localizes to telomeres by binding to the telomeric repeat binding factor 1 (TRF1) through its ankyrin repeats. Tankyrase exhibits PARP activity functioning as acceptors for adenosine diphosphate (ADP)-ribosylation. Since ADP-ribosylation of TRF1 diminishes its ability to bind to telomeric DNA, this suggests that telomere function in human cells is regulated by poly (ADP)-ribosylation. Both the cell cycle and TRF1 may regulate the subcellular localization of tankyrase. The cDNA coding for TIN2 protein was isolated as interacting partner of TRF1 from a yeast two-hybrid cDNA library screening. Tin2 localizes to telomeres and is essential for proper regulation of telomere length. TIN2, like TRF1, is widely and constitutively expressed, suggesting that these proteins act together to counterbalance telomere elongation by telomerase. However, endogenous TIN2, like endogenousTRF1, is not highly expressed, and therefore may be difficult to visualize by immunostaining in certain cell systems.

Specifications

• Antigen: TIN2
• Applications: ELISA, Western Blot
• Classification: Recombinant Monoclonal
• Clone: 4W5P2
• Concentration: 1.14 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 0.05% BSA, 50% glycerol and 0.02% sodium azide; pH 7.3
• Gene: Tinf2
• Gene Accession No.: Q9BSI4, Q9QXG9
• Gene Alias: AW552114; D14Wsu146e; DKCA3; TERF1 (TRF1)-interacting nuclear factor 2; TERF1 interacting nuclear factor 2; TERF1-interacting nuclear factor 2; tin; TIN2; Tin-2; TINF2; TRF1-interacting nuclear factor 2; TRF1-interacting nuclear protein 2
• Gene Symbols: Tinf2
• Host Species: Rabbit
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 210-309 of human TIN2/TINF2 (Q9BSI4).
• Purification Method: Affinity Chromatography
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 26277, 28113, 290232
• Target Species: Human, Mouse, Rat
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG