Invitrogen™ TLR2 Chimeric Recombinant Rabbit Monoclonal Antibody (TL2.1)

Catalog No.	Quantity
PIMA548249	200 μg

Catalog No. PIMA548249 Supplier Invitrogen™ Supplier No. MA548249

Description

Rabbit Recombinant Monoclonal Antibody

Specificity: This antibody is specific to TLR2 (extracellular domain) and does not crossreact with human TLR1 or TLR4. TLR2 is involved in the immunological response against bacterial lipoproteins and other microbial cell wall components.

TLR2 is a member of the Toll-like receptor (TLR) family which play a fundamental role in pathogen recognition and activation of innate immunity. TLR proteins act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors such as Protein Kinase C (PKC) alpha/beta and NF-kappa-B. TLR2 can form heterodimers with either TLR1 or TLR6, and as a heterodimer, can recognize a variety of bacterial and mycoplasma lipoproteins respectively. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. The various TLRs exhibit different patterns of expression. TLR2 is expressed most abundantly in peripheral blood leukocytes, and mediates host response to Gram-positive bacteria and yeast via stimulation of NF-kappaB. TLR2 aids in the recognition of pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. Ten human homologs of TLRs (TLR1-10) have been described. TLR2 is expressed in human cells such as tonsils, microglia, lymph nodes, and appendices, activated B-cells in germinal centers. CD14+ monocytes express the highest level of TLR2 followed by CD15+ granulocytes, CD19+ B-cells, and CD3+ T-cells. The expression of TLR2 on different cell types are regulated by different immune response modifiers. For example, LPS, GM-CSF, IL-1, and IL-10 up regulates TLR2 whereas IL-4, IFN-gamma, and TNF down regulate TLR2 expression in monocytes.

Specifications

• Antigen: TLR2 Chimeric
• Applications: Flow Cytometry, Inhibition Assays, Immunoprecipitation, Western Blot, Immunocytochemistry
• Classification: Recombinant Monoclonal
• Clone: TL2.1
• Concentration: 1 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 0.02% ProClin 300
• Gene: Tlr2
• Gene Accession No.: O60603
• Gene Alias: CD282; Ly105; TIL4; Tlr2; TLR-2; toll like receptor 2; toll/interleukin-1 receptor-like protein 4; Toll-like receptor; toll-like receptor 2; toll-like receptor 2 variant 1; toll-like receptor 2 variant 2; toll-like-receptor 2, exon 1a_; Toll-like-receptor, exon 1b; Toll-like-receptor, exon 1b_; Toll-like-receptor2, exon 1a
• Gene Symbols: Tlr2
• Host Species: Rabbit
• Immunogen: This antibody was raised by immunising BALB/c mice with CHO cells transfected with human TLR2 cDNA.
• Purification Method: Protein A
• Quantity: 200 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 7097
• Target Species: Human
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG κ