TLR4 Antibody (76B357.1) - Azide Free, Novus Biologicals™

Mouse Monoclonal Antibody has been used in 25 publications

Catalog Number	Mfr. No.	Quantity
NBP227149SS	Novus Biologicals NBP227149SS	0.025 mg
NBP227149	Novus Biologicals NBP227149	0.1 mg

Description

TLR4 Monoclonal specifically detects TLR4 in Human, Mouse, Rat, Porcine, Bovine, Mammal samples. It is validated for Western Blot, Chromatin Immunoprecipitation, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Flow (Cell Surface), Flow (Intracellular), CyTOF-ready, Knockout Validated, Knockdown Validated, Block/Neutralize.

Specifications

• Antigen: TLR4
• Concentration: 1.0 mg/ml
• Applications: Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), KnockDown, Neutralization
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Adaptive Immunity, Cytokine Research, Immunology, Innate Immunity, Signal Transduction, Toll Like Receptors
• Gene Accession No.: O00206
• Gene ID (Entrez): 7099
• Immunogen: This antibody was developed against a portion of amino acids 100-200 of human TLR4 (NP_612564).
• Primary or Secondary: Primary
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

• Clone: 76B357.1
• Dilution: Western Blot 1-3 ug/ml, Flow Cytometry 1ul/1 million cells, Immunohistochemistry, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry-Paraffin 5ug/ml, Immunohistochemistry-Frozen 1:500, Flow (Cell Surface) reported in multiple pieces of scientific literature, Flow (Intracellular) reported in scientific literature (PMID 18577706), Chromatin Immunoprecipitation (ChIP) 1:10-1:500, CyTOF-ready, Knockout Validated, KnockDown Validated, Block/Neutralize reported in scientific literature (PMID 26063617)
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human, Mouse, Rat, Pig, Bovine, Mammalia
• Gene Alias: ARMD10, CD284, CD284 antigen, hTollhomolog of Drosophila toll, TOLL, toll-like receptor 4
• Gene Symbols: TLR4
• Isotype: IgG2b κ
• Purification Method: Protein G purified
• Test Specificity: The amino acid sequence used has 100% identity in human, chimp, baboon, Chinese hamster, and rat; 93% identity in bovine and pig; 85% identity in sheep and 78% identity in cat, horse and mouse.