TLR4 Antibody, Novus Biologicals™

Rabbit Polyclonal Antibody has been used in 22 publications

Supplier:  Novus Biologicals NB10056580SS

Catalog No. NB10056580T

Description

TLR4 Polyclonal specifically detects TLR4 in Human, Mouse, Rat, Rhesus Macaque samples. It is validated for Western Blot, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Proximity Ligation Assay.

Specifications

• Antigen: TLR4
• Classification: Polyclonal
• Conjugate: Unconjugated
• Formulation: PBS with 0.05% Sodium Azide
• Gene Alias: ARMD10, CD284, CD284 antigen, hTollhomolog of Drosophila toll, TOLL, toll-like receptor 4
• Host Species: Rabbit
• Purification Method: Immunogen affinity purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: This antibody was tested against partial recombinant mouse TLR4 (extracellular portion), and a 75-80 kDa band was observed. Full-length TLR4 is observed at approximately 90 kD. 2. Zager et al (2006, Fig. 10) observed TLR4 as an ∼95 kDa doublet which is thought to represent different degrees of glycosylation. See Product Citation (Renal Physiol 291:F546-F556) for additional information. 3. Presumptive TLR4 30 and 60 kDa cleavage fragments are described in Zagar et al 2006.
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Isotype: IgG

• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), In situ PLA
• Concentration: 1.0 mg/ml
• Dilution: Western Blot 2 - 5 ug/mL, Immunohistochemistry, Immunocytochemistry/Immunofluorescence 2 - 5 ug/mL, Immunohistochemistry-Paraffin 5 ug/mL, Proximity Ligation Assay
• Gene Accession No.: O00206
• Gene Symbols: TLR4
• Immunogen: This antibody was developed against a sythetic peptide corresponding to amino acids in a range between 30-80 of mouse TLR4.
• Quantity: 0.025 mg
• Research Discipline: Adaptive Immunity, Cytokine Research, Immunology, Innate Immunity, Signal Transduction
• Gene ID (Entrez): 7099
• Target Species: Human, Mouse, Rat, Rhesus Macaque
• Form: Purified

For Research Use Only