Novus Biologicals™ TLR4 Inhibitor Peptide Set

Catalog No.	Quantity
NBP226244	2 Vials

Catalog No. NBP226244 Supplier Novus Biologicals™ Supplier No. NBP226244

Description

Applications: Flow Cytometry, Functional, In vitro assay, Binding Inhibition, Block/Neutralize

• Consists of 11 amino acid (KYSFKLILAEY) inhibitory sequence, derived from A46 vaccinia virus protein, linked to 9R (RRRRRRRRR) homopolymer delivery sequence

Specifications

• Host Species: Human, Mouse, Rat
• Color: White
• Components: VIPER: A TLR4 Inhibitory Peptide: 1mg (lyophilized) KYSFKLILAEYRRRRRRRRR (VIPER sequence: KYSFKLILAEY). Molecular weight: 2780.3CP7, Control Peptide: 1mg (lyophilized) RNTISGNIYSARRRRRRRRR (Control sequence: RNTISGNIYSA). Molecular weight: 2601
• For Use With (Application): The inhibitor is used in assays to inhibit TLR4 activation; see Figure 3 and also refer to Lysakova-Devine et al (2010) for examples. Optimal inhibitor concentrations should be established through titration and may vary between model systems. We recommend an initial titration from 0-30 uM for in vitro assays (Fig 3). Control concentrations should mirror inhibitor concentrations. Inhibitor and control should be preincubated with cells prior to ligand activation to allow sufficient time for the peptides to enter from the media into the cell. We typically preincubate with inhibitor and control peptides for 2 h prior to TLR4 activation with LPS (Fig 3); however, optimal preincubation times may vary between model systems. The TLR4/MD-2/CD14 stably transfected cell line is a useful positive control model system for studying inhibition of TLR4 activation by VIPER (Fig 3). SEAP is used as a readout assay in Figure 3 to measure TLR4 inhibition. A novel model system is shown in Figure 1 where TLR4 inhibitor peptide, but not CP7, inhibited TLR4 activation in Mal-deficient immortalized mouse bone marrow-derived macrophages (iBMDMs). In these iBMDMs, the inhibitor targets TLR4-TRAM, but not TLR-Mal, interactions as Mal is not expressed. TNF-alpha is used as a readout assay in Figure 1 to measure inhibition. Use in Function reported in scientific literature (PMID 24630524) Use in blocking neutralizing reported in multiple pieces of scientific literature FLOW application reported by Alvarez et al - Conference on Retroviruses and Opportunistic Infections - CROI 2014 (Abstract: The NCOR2-Nurr1-CoREST Transrepression Axis Impairs HIV Reactivation in Latently Infected Microglial Cells) Use in Binding inhibition reported in scientific literature (PMID 27897392).
• Content And Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
• Quantity: 2 Vials
• Product Type: TLR4 Inhibitor Peptide Set
• Molecular Weight (g/mol): 2780.3
• Inhibitors: TLR4
• Form: Lyophilized Solid

For Research Use Only