TLR4/MD-2 Complex Monoclonal Antibody (MTS510), Functional Grade, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
16-992-482	Invitrogen™ 16992482	100 μg

Description

Description: The MTS510 monoclonal antibody reacts with the mouse Toll-like receptor 4 (TLR4)/MD-2 complex. At least ten members of the Toll family have been identified. This family of type I transmembrane proteins is characterized by an extracellular domain with leucine-rich repeats and a cytoplasmic domain with homology to the type I IL-1 receptor. Two of these receptors, TLR2 and TLR4, are pattern recognition receptors and signaling molecules in response to bacterial lipoproteins and have been implicated in innate immunity and inflammation. TLR4 physically associates with MD-2, and together with CD14, this complex is responsible for LPS recognition and signaling. In the mouse, TLR4 is expressed by thioglycolate-elicited peritoneal macrophages. Incubation of peritoneal macrophages with LPS results in down regulation of surface TLR4/MD-2. The TLR4 gene is defective in C3H/HeJ and C57BL/10ScCr mice, both of which have been well characterized as hyporesponders to LPS. The MTS510 monoclonal antibody co-immunoprecipitates MD-2 (∽30 kDa) and TLR4 (∽100 kDa), and preferentially reacts with TLR4 that is associated with MD-2. In comparison, binding of the UT41 monoclonal antibody occurs with and without formation of the TLR4/MD-2 complex. Please contact eBioscience Technical Support for further information. Applications Reported: MTS510 has been reported for use in flow cytometric analysis and inhibition of LPS-induced cytokine production.

MD-2 is a member of the Toll/interleukin-1 receptor (TIR) family, a group of proteins that include the Toll-like receptors (TLRs). TLRs are signaling molecules that recognize different pathogen-associated molecular patterns (PAMPs) and serve as an important link between the innate and adaptive immune responses. TLR4, the major signaling receptor for lipopolysaccharide (LPS), requires the binding of MD-2 to its extracellular region for maximal response to LPS. The specificity of this response is determined by the species of MD-2; e.g., human MD-2 transfected into mouse cells can cause mouse TLR4 to react to LPS analogs that are normally antagonistic to human but not mouse TLR4.

Specifications

• Antigen: TLR4/MD-2 Complex
• Concentration: 1 mg/mL
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Formulation: PBS with no preservative; pH 7.2
• Gene ID (Entrez): 17087, 21898
• Isotype: IgG2a κ
• Purification Method: Affinity Chromatography
• Product Type: Antibody

• Clone: MTS510
• Applications: Flow Cytometry, Functional Assay, Neutralization
• Conjugate: Functional Grade
• Host Species: Rat
• Target Species: Mouse
• Gene Accession No.: Q9JHF9, Q9QUK6
• Gene Symbols: Ly96, Tlr4
• Primary or Secondary: Primary
• Content And Storage: 4°C

For Research Use Only.