Invitrogen™ Tmem119 Monoclonal Antibody (V3RT1GOsz), Brilliant Violet™ 421, eBioscience™

Rat Monoclonal Antibody

Supplier:  Invitrogen™ 404611982

Catalog No. 40-461-1982

Description

Description: This V3RT1GOsz monoclonal antibody recognizes mouse Tmem119. This clone has been reported to be successfully used in flow cytometry. Applications Reported: This V3RT1GOsz antibody has been reported for use in flow cytometric analysis. Applications Tested: This V3RT1GOsz antibody has been tested by flow cytometric analysis of mouse brain cells after myelin removal. This may be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Brilliant Violet™ 421 (BV421) is a dye that emits at 423 nm and is intended for use on cytometers equipped with a violet (405 nm) laser. Please make sure that your instrument is capable of detecting this fluorochrome. When using two or more Super Bright, Brilliant Violet™, Brilliant Ultra Violet™, or other polymer dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer or Brilliant Stain Buffer for more information. Excitation: 407 nm; Emission: 423 nm; Laser: Violet Laser. ...

Tmem119 encodes a single-pass type I transmembrane protein which seems to interact with a multitude of micro-RNAs as well as certain transcription factors such as SMAD1, SMAD5 and RUNX2. Tmem119 has been identified as a surface marker of microglia that can be used to reliably distinguish microglia from infiltrating macrophages. Microglial cells are phagocytes that are restricted to the brain and involved in clearing up debris and dendrite pruning of neurons. They display a high functional plasticity, balancing neuroprotective and neurotoxic functions, which is reflected by their distinct physiology in response to changes in their microenvironment. Microglia are particularly mobile in areas of the brain undergoing restructuring. Reactive microglia co-express the marker Iba1. In a pathological context, microglia have been associated with differentiation of pathogenic/inflammatory A1 type astrocytes by secretion of Cq1, IL-1a and TNF when stimulated with LPS, and thought to contribute to the pathology of several neurodegenerative diseases such as Alzheimers, Parkinsons, Chorea Huntingtons disease and Multiple Sclerosis. The specific function Tmem119 plays on microglial cells remains unclear. Outside of the neural system, Tmem119 seems to be involved in osteoblast differentiation/ossification.

Specifications

• Antigen: Tmem119
• Classification: Monoclonal
• Concentration: 0.2 mg/mL
• Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
• Gene Accession No.: Q8R138
• Gene Symbols: TMEM119
• Immunogen: cell surface peptide.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG2a κ

• Applications: Flow Cytometry
• Clone: V3RT1GOsz
• Conjugate: Brilliant Violet 421
• Gene: TMEM119
• Gene Alias: AW208946; BC025600; OBIF; Osteoblast induction factor; PSEC0199; Tmem119; Transmembrane protein 119; UNQ731/PRO1415
• Host Species: Rat
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 231633
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Form: Liquid