Invitrogen™ TMEM119 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA5120705

Catalog No. PIPA5120705

Description

Positive test controls include: Mouse liver, Mouse brain, Rat brain. Immunogen sequence: SRPEEALDSS RQLQADILAA TQNLKSPTRA ALGGGDGARM VEGRGAEEEE KGSQEGDQEV QGHGVPVETP EAQEEPCSGV LEGAVVAGEG QGELEGSLLL AQEAQGPVGP P.

Tmem119 encodes a single-pass type I transmembrane protein which seems to interact with a multitude of micro-RNAs as well as certain transcription factors such as SMAD1, SMAD5 and RUNX2. Tmem119 has been identified as a surface marker of microglia that can be used to reliably distinguish microglia from infiltrating macrophages. Microglial cells are phagocytes that are restricted to the brain and involved in clearing up debris and dendrite pruning of neurons. They display a high functional plasticity, balancing neuroprotective and neurotoxic functions, which is reflected by their distinct physiology in response to changes in their microenvironment. Microglia are particularly mobile in areas of the brain undergoing restructuring. Reactive microglia co-express the marker Iba1. In a pathological context, microglia have been associated with differentiation of pathogenic/inflammatory A1 type astrocytes by secretion of Cq1, IL-1a and TNF when stimulated with LPS, and thought to contribute to the pathology of several neurodegenerative diseases such as Alzheimers, Parkinsons, Chorea Huntingtons disease and Multiple Sclerosis. The specific function Tmem119 plays on microglial cells remains unclear. Outside of the neural system, Tmem119 seems to be involved in osteoblast differentiation/ossification.

Specifications

• Antigen: TMEM119
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: TMEM119
• Gene Alias: AW208946; BC025600; OBIF; Osteoblast induction factor; PSEC0199; Tmem119; Transmembrane protein 119; UNQ731/PRO1415
• Host Species: Rabbit
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 231633, 304581
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: ELISA, Western Blot
• Concentration: 0.73 mg/mL
• Formulation: PBS with 50% glycerol and 0.01% thimerosal; pH 7.3
• Gene Accession No.: Q8R138
• Gene Symbols: TMEM119
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 160-270 of human TMEM119 (NP_859075.2).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Mouse, Rat
• Product Type: Antibody
• Isotype: IgG