Invitrogen™ TNF alpha Monoclonal Antibody (4F4)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MP390

Catalog No. ENMP390

Description

MP390 detects natural and recombinant TNF alpha, with a predicted molecular weight of approximately 25 kDa. This antibody will not cross-react with human or mouse TNFa, mouse TNFb, or porcine TNFb. This is a carrier-free low endotoxin formulation, <1.0 EU/mg. This product can be used as a coating antibody in a sandwich ELISA (100 μg/well) in combination with biotin-labeled TNFa monoclonal antibody (Product # M302B, 0.1-0.65 μg/mL). For Western blot, this antibody has been successfully used in conjunction with 0.02 μg/mL Biotin-conjugated Goat-anti-Mouse IgG (Product # 31800), 0.05 μg/mL HRP-conjugated Streptavidin (Product # N100), and recombinant Porcine TNFa (Product # RPTNFAI), followed by visualization using SuperSignal™ West Pico Chemiluminescent Substrate (Product # 34080). This product has been tested for endotoxins by limulus amoebocyte lysate (LAL) assay and contains an endotoxin concentration of less than or equal to 10 endotoxin units per milligram (EU/mg).

TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.

Specifications

• Antigen: TNF alpha
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with no preservative
• Gene Accession No.: P23563
• Gene Symbols: Tnf
• Immunogen: Recombinant porcine TNF alpha (Baculovirus-derived).
• Quantity: 500 μg
• Primary or Secondary: Primary
• Target Species: Pig
• Product Type: Antibody
• Isotype: IgG1

• Applications: ELISA, Western Blot
• Clone: 4F4
• Conjugate: Unconjugated
• Gene: Tnf
• Gene Alias: APC1 protein; Cachectin; C-domain 1; C-domain 2; cTNF; DADB-70P7.1; DIF; ICD1; ICD2; Intracellular domain 1; Intracellular domain 2; N-terminal fragment; NTF; RATTNF; Tnf; TNF alpha; TNF superfamily; TNF a; TNF, macrophage-derived; TNF, monocyte-derived; TNFA; TNF-a; TNFalpha; TNF-alpha; Tnfsf1a; TNFSF2; TNFa; TNLG1F; Tumor necrosis factor; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; tumor necrosis factor alpha (cachetin); tumor necrosis factor alpha precursor; tumor necrosis factor ligand 1F; tumor necrosis factor ligand superfamily member 2; Tumor necrosis factor, membrane form; Tumor necrosis factor, soluble form; tumor necrosis factor-alpha; tumor necrosis factor-alpha precursor; tumor-necrosis factor; tumour necrosis factor
• Host Species: Mouse
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 397086
• Content And Storage: -20°C
• Form: Liquid

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov