Invitrogen™ TNF alpha Monoclonal Antibody (TN3-19.12), Biotin, Functional Grade, eBioscience™

Catalog No.	Quantity
50-157-02	500 μg

Catalog No. 50-157-02 Supplier Invitrogen™ Supplier No. 36742385

Description

Armenian Hamster Monoclonal Antibody

The TN3-19.12 monoclonal antibody reacts with mouse, rat, and rabbit tumor necrosis factor-alpha (TNF-alpha), but not with human TNF-alpha. The TN3-19.12 antibody is a neutralizing antibody. mouse TNF-alpha is a 17 kDa factor produced by macrophages, monocytes, neutrophils, CD4+ T cells and NK cells. A 26 kDa form of TNF-alpha is expressed as a membrane-bound molecule. TNF-alpha is cytolytic and plays an important role in immune regulation. Dimers and trimers of TNF-alpha have been observed.Applications Tested TestedThe Functional Grade Biotin TN3-19 antibody has been tested by LAL assay to ensure it is free of endotoxin, and has been tested as the detection antibody in a sandwich ELISA for analysis of mouse TNF alpha in combination with the purified polyclonal anti-mouse TNF alpha antibody (Product # 14-7341-85) for capture and recombinant mouse TNF alpha (Product # 14-8321-62) as the standard. A suitable range of concentrations of the FG Biotin TN3-19 antibody for ELISA detection is 0.5-2 μg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2000 pg/mL - 15.0 pg/mL should be included in each ELISA plate.Storage and handling: Use in a sterile environment.

TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.

Specifications

• Antigen: TNF alpha
• Applications: Functional Assay
• Classification: Monoclonal
• Clone: TN3-19.12
• Concentration: 0.5 mg/mL
• Conjugate: Biotin, Functional Grade
• Formulation: PBS with no preservative; pH 7.2
• Gene: Tnf
• Gene Accession No.: P06804, P16599
• Gene Alias: APC1 protein; Cachectin; C-domain 1; C-domain 2; cTNF; DADB-70P7.1; DIF; ICD1; ICD2; Intracellular domain 1; Intracellular domain 2; N-terminal fragment; NTF; RATTNF; Tnf; TNF alpha; TNF superfamily; TNF α; TNF, macrophage-derived; TNF, monocyte-derived; TNFA; TNF-a; TNFalpha; TNF-alpha; Tnfsf1a; TNFSF2; TNFα; TNLG1F; Tumor necrosis factor; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; tumor necrosis factor alpha (cachetin); tumor necrosis factor alpha precursor; tumor necrosis factor ligand 1F; tumor necrosis factor ligand superfamily member 2; Tumor necrosis factor, membrane form; Tumor necrosis factor, soluble form; tumor necrosis factor-alpha; tumor necrosis factor-alpha precursor; tumor-necrosis factor; tumour necrosis factor
• Gene Symbols: Tnf
• Host Species: Armenian Hamster
• Purification Method: Affinity chromatography
• Quantity: 500 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 21926, 24835
• Target Species: Mouse, Rat
• Content And Storage: 4°C
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG