Invitrogen™ TNF alpha Polyclonal Antibody

Catalog No.	Quantity
AMC3012	500 μg

Catalog No. AMC3012 Supplier Invitrogen™ Supplier No. AMC3012

Description

Rabbit Polyclonal Antibody

Reconstitute using 0.5 mL sterile distilled water.

TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.

Specifications

• Antigen: TNF alpha
• Applications: ELISA, Neutralization, Western Blot, Immunocytochemistry
• Classification: Polyclonal
• Concentration: 1 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 125mM trehalose and no preservative
• Gene: Tnf
• Gene Accession No.: P01375, P06804
• Gene Alias: APC1 protein; Cachectin; C-domain 1; C-domain 2; cTNF; DADB-70P7.1; DIF; ICD1; ICD2; Intracellular domain 1; Intracellular domain 2; N-terminal fragment; NTF; RATTNF; Tnf; TNF alpha; TNF superfamily; TNF α; TNF, macrophage-derived; TNF, monocyte-derived; TNFA; TNF-a; TNFalpha; TNF-alpha; Tnfsf1a; TNFSF2; TNFα; TNLG1F; Tumor necrosis factor; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; tumor necrosis factor alpha (cachetin); tumor necrosis factor alpha precursor; tumor necrosis factor ligand 1F; tumor necrosis factor ligand superfamily member 2; Tumor necrosis factor, membrane form; Tumor necrosis factor, soluble form; tumor necrosis factor-alpha; tumor necrosis factor-alpha precursor; tumor-necrosis factor; tumour necrosis factor
• Gene Symbols: Tnf
• Host Species: Rabbit
• Immunogen: Recombinant mouse TNF-alpha produced in E. coli.
• Purification Method: Protein A
• Quantity: 500 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 21926, 7124
• Target Species: Human, Mouse
• Content And Storage: 4°C
• Product Type: Antibody
• Form: Lyophilized
• Isotype: Ig

Frequently Asked Questions (FAQs)

Which antibodies directed to cytokines are suitable for use in immunohistochemistry with paraffin sections?

The following antibodies have been used successfully for IHC with paraffin sections:
Mouse (monoclonal) Anti-Human TGF-?1 (Cat. No. AHG0051), Rat (monoclonal) Anti-Human Interleukin-13 (Cat. No. AHC0132), Rabbit (polyclonal) Anti-Human IL-8 (Cat. No. AHC0881), Rabbit (polyclonal) Anti-Mouse Interferon-? (Cat. No. AMC4034), Goat (polyclonal) Anti-Mouse TNF-? (Cat. No. AMC3012), and Rabbit (polyclonal) Anti-Rat TNF-? (Cat. No. ARC3012).

You can also locate these and other antibodies with our helpful Primary Antibody Selection Guide. This can be accessed by searching "Primary Antibody Selector Tool" on our website. Once you've reached the Selection Guide, you can narrow the search by clicking on your desired links at the top of the Guide.