TNF alpha Polyclonal Antibody, Rockland™

Rabbit Polyclonal Antibody

Catalog Number	Mfr. No.	Quantity
50-199-0265	Rockland Immunochemicals 110401321S	25 μL
50-199-0264	Rockland Immunochemicals 1104013210100	100 μL
50-199-0263	Rockland Immunochemicals 110401321	1 mL

Description

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. The antiserum is directed against mature 17,000 MW mouse TNFa and is useful in determining its presence in various assays. The antibody does not recognize mouse TNFb (lymphotoxin). This antiserum will recognize the cell-bound precursor of TNFa as a 26,000 protein in immunoblots, particularly in denatured samples. This antibody is also useful for neutralization of mouse activity in bioassays. It does not neutralize the biological activity of lymphotoxin. For neutralization, it is recommended to incubate the sample with a 1:200 dilution of the antibody for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended.

TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.

Specifications

• Antigen: Mouse TNF alpha
• Applications: ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
• Conjugate: Unconjugated
• Host Species: Rabbit
• Target Species: Mouse
• Gene Accession No.: P06804
• Gene ID (Entrez): 21926
• Immunogen: The whole rabbit serum was prepared by repeated immunizations with recombinant mouse TNFa produced in E.coli.
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Gene: Tnf

• Concentration: 85 mg/mL
• Classification: Polyclonal
• Form: Liquid
• Regulatory Status: RUO
• Formulation: Antibody with no preservative; pH
• Gene Alias: APC1 protein; BOS_21587; Cachectin; C-domain 1; C-domain 2; cTNF; DADB-70P7.1; DIF; ICD1; ICD2; Intracellular domain 1; Intracellular domain 2; N-terminal fragment; NTF; RATTNF; SBAB-548A10.2; Tnf; TNF alpha; TNF superfamily; TNF a; TNF, macrophage-derived; TNF, monocyte-derived; Tnfa; TNF-a; TNFalpha; TNF-alpha; Tnfsf1a; Tnfsf2; TNFa; TNLG1F; Tumor necrosis factor; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; tumor necrosis factor alpha (cachetin); tumor necrosis factor ligand 1F; Tumor necrosis factor ligand superfamily member 2; Tumor necrosis factor, membrane form; Tumor necrosis factor, soluble form; tumor necrosis factor-alpha; tumor-necrosis factor; tumour necrosis factor
• Gene Symbols: Tnf
• Primary or Secondary: Primary
• Product Type: Antibody