Invitrogen™ TNF beta Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ 500P31BBT500UG

Catalog No. 50-271-8512

Description

AA Sequence of recombinant protein: MLPGVGLTPS AAQTARQHPK MHLAHSTLKP AAHLIGDPSK QNSLLWRANT DRAFLQDGFS LSNNSLLVPT SGIYFVYSQV VFSGKAYSPK ATSSPLYLAH EVQLFSSQYP FHVPLLSSQK MVYPGLQEPW LHSMYHGAAF QLTQGDQLST HTDGIPHLVL SPSTVFFGAF AL. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human TNF-beta. Anti-Human TNF-beta-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect Human TNF-beta by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human TNF-beta (500-P31B) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human TNF-beta. Western Blot: To detect Human TNF-beta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human TNF-beta is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P31BBT-1MG will be provided as 2 x 500 μg

TNF beta, a member of the tumor necrosis factor family, is a cytokine produced by lymphocytes. The protein is highly inducible, secreted, and forms heterotrimers with lymphotoxin-beta which anchor lymphotoxin-alpha to the cell surface. This protein also mediates a large variety of inflammatory, immunostimulatory, and antiviral responses, is involved in the formation of secondary lymphoid organs during development and plays a role in apoptosis. Genetic variations in this gene are associated with susceptibility to leprosy type 4 and psoriatic arthritis.

Specifications

• Antigen: TNF beta
• Classification: Polyclonal
• Conjugate: Biotin
• Gene: LTA
• Gene Alias: DAMA-25N12.13; hlb382; hypothetical protein; LT; LT[a]; LT-[a]; LTA; LTalpha; LT-alpha; Ltx; lymphotoxin A; lymphotoxin alpha; lymphotoxin alpha (TNF superfamily, member 1); lymphotoxin-alpha; lymphotoxin-alpha; lymphotoxin alpha; TNF b; TNF beta; TNF superfamily, member 1; TNF ß; Tnfb; TNFbeta; TNF-beta; TNF-N; Tnfsf1; Tnfsf1b; TNFß; TNLG1E; tumor necrosis factor beta; tumor necrosis factor ligand 1E; tumor necrosis factor ligand superfamily member 1
• Host Species: Rabbit
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 4049
• Content And Storage: -20°C
• Form: Lyophilized

• Applications: ELISA, Western Blot
• Concentration: 0.1-1.0 mg/mL
• Formulation: PBS with no preservative
• Gene Accession No.: P01374
• Gene Symbols: LTA
• Immunogen: E.coli-derived, 18.6 kDa Recombinant Human TNF-beta.
• Quantity: 500 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG