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TNF Mouse anti-Human, Rhesus, Cynomolgus, Baboon, Brilliant Violet 750, Clone: MAb11, BD

Mouse Monoclonal Antibody

Manufacturer:  BD Biosciences 566359

Catalog No. BDB566359

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The MAb11 monoclonal antibody specifically binds to human tumor necrosis factor (TNF, also known as TNF- ) protein. TNF is an efficient juxtacrine, paracrine and endocrine mediator of inflammatory and immune functions. It regulates the growth and differentiation of a variety of cell types. TNF is cytotoxic for transformed cells when in conjunction with IFN- . It is secreted by activated monocytes/macrophages and other cells such as B cells, T cells and fibroblasts. The immunogen used to generate the MAb11 hybridoma was recombinant human TNF. The MAb11 antibody has been reported to crossreact with Rhesus Macaque TNF.

The antibody was conjugated to BD Horizon BV750 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 750nm. BD Horizon Brilliant BV750 can be excited by the violet laser (405 nm) and detected with a 750/30 nm filter with a 740 nm long pass. Due to spectral differences between labeled cells and beads, using BD™ CompBeads can result in incorrect spillover values when used with BD Horizon BV750 reagents. Therefore, the use of BD CompBeads or BD CompBeads Plus to determine spillover values for these reagents is not recommended. BV750


Aqueous buffered solution containing ≤0.09% sodium azide.
Tumor necrosis factor alpha, TNF-a, TNF-α, TNFSF2, Cachectin
Recombinant Human TNF
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV750 under optimum conditions, and unconjugated antibody and free BD Horizon BV750 were removed.
Baboon, Cynomolgus, Human, Rhesus Monkey
Brilliant Violet 750
Affinity Purified
Mouse IgG1, κ
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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