Invitrogen™ TNFRSF11B Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ 500P149BT500UG

Catalog No. 50-271-8666

Description

AA Sequence of recombinant protein: METFPPKYLH YDEETSHQLL CDKCPPGTYL KQHCTAKWKT VCAPCPDHYY TDSWHTSDEC LYCSPVCKEL QYVKQECNRT HNRVCECKEG RYLEIEFCLK HRSCPPGFGV VQAGTPERNT VCKRCPDGFF SNETSSKAPC RKHTNCSVFG LLLTQKGNAT HDNICSGNSE STQK. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human OPG. Anti-Human OPG-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect Human OPG by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human OPG (500-P149) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human OPG. Western Blot: To detect hOPG by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hOPG is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P149BT-1MG will be provided as 2 x 500 μg

The protein encoded by this gene is a member of the TNF-receptor superfamily. This protein is an osteoblast-secreted decoy receptor that functions as a negative regulator of bone resorption. This protein specifically binds to its ligand, osteoprotegerin ligand, both of which are key extracellular regulators of osteoclast development. Studies of the mouse counterpart also suggest that this protein and its ligand play a role in lymph-node organogenesis and vascular calcification. Alternatively spliced transcript variants of this gene have been reported, but their full length nature has not been determined.

Specifications

• Antigen: TNFRSF11B
• Classification: Polyclonal
• Conjugate: Biotin
• Gene: TNFRSF11B
• Gene Alias: LOW QUALITY PROTEIN: tumor necrosis factor receptor superfamily member 11B; MGC29565; OCIF; Opg; osteoclastogenesis inhibitory factor; Osteoprotegerin; PDB5; soluble OPG; soluble Osteoprotegerin; sOPG; TNF receptor superfamily member 11b; TNFRSF11B; TR1; tumor necrosis factor receptor superfamily member 11B; tumor necrosis factor receptor superfamily, member 11b; tumor necrosis factor receptor superfamily, member 11b (osteoprotegerin)
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 4982
• Content And Storage: -20°C
• Form: Lyophilized

• Applications: ELISA, Western Blot
• Concentration: 0.1-1.0 mg/mL
• Formulation: PBS with no preservative
• Gene Accession No.: O00300
• Gene Symbols: TNFRSF11B
• Immunogen: E.coli-derived, 20.0 kDa Recombinant Human OPG.
• Quantity: 500 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG