TNFRSF11B Polyclonal Antibody, PeproTech®, Invitrogen™

Rabbit Polyclonal Antibody

Catalog Number	Mfr. No.	Quantity
500P1491MG	Pepro Tech Inc 500P1491MG	1 mg

Description

AA Sequence of recombinant protein: METFPPKYLH YDEETSHQLL CDKCPPGTYL KQHCTAKWKT VCAPCPDHYY TDSWHTSDEC LYCSPVCKEL QYVKQECNRT HNRVCECKEG RYLEIEFCLK HRSCPPGFGV VQAGTPERNT VCKRCPDGFF SNETSSKAPC RKHTNCSVFG LLLTQKGNAT HDNICSGNSE STQK. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human OPG. Anti-Human OPG-specific antibody was purified by affinity chromatography employing an immobilized Human OPG matrix. Sandwich ELISA: To detect Human OPG by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human OPG (500-P149Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human OPG. Western Blot: To detect Human OPG by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human OPG is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

The protein encoded by this gene is a member of the TNF-receptor superfamily. This protein is an osteoblast-secreted decoy receptor that functions as a negative regulator of bone resorption. This protein specifically binds to its ligand, osteoprotegerin ligand, both of which are key extracellular regulators of osteoclast development. Studies of the mouse counterpart also suggest that this protein and its ligand play a role in lymph-node organogenesis and vascular calcification. Alternatively spliced transcript variants of this gene have been reported, but their full length nature has not been determined.

Specifications

• Antigen: TNFRSF11B
• Applications: ELISA, Western Blot
• Conjugate: Unconjugated
• Host Species: Rabbit
• Target Species: Human
• Gene Accession No.: O00300
• Gene ID (Entrez): 4982
• Immunogen: E.coli-derived Recombinant Human OPG.
• Purification Method: Antigen Affinity Chromatography
• Gene: TNFRSF11B

• Concentration: 0.1-1.0 mg/mL
• Classification: Polyclonal
• Form: Lyophilized
• Regulatory Status: RUO
• Formulation: PBS with no preservative
• Gene Alias: LOW QUALITY PROTEIN: tumor necrosis factor receptor superfamily member 11B; MGC29565; OCIF; Opg; osteoclastogenesis inhibitory factor; Osteoprotegerin; PDB5; soluble OPG; soluble Osteoprotegerin; sOPG; TNF receptor superfamily member 11b; TNFRSF11B; TR1; tumor necrosis factor receptor superfamily member 11B; tumor necrosis factor receptor superfamily, member 11b; tumor necrosis factor receptor superfamily, member 11b (osteoprotegerin)
• Gene Symbols: TNFRSF11B
• Primary or Secondary: Primary
• Content And Storage: -20°C