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Invitrogen™ TO-PRO™3 Ready Flow™ Reagent

Catalog No. R37170
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R37170 120 assays
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Catalog No. R37170 Supplier Invitrogen™ Supplier No. R37170
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Description

TO-PRO™3 Ready Flow™ Reagent is a bright, easy-to-use nucleic acid stain for distinguishing dead cells from live cells in flow cytometry assays.

  • A bright and easy-to-use nucleic acid stain for distinguishing dead cells from live cells in flow cytometry assays
  • Dye has excitation/emission maxima of 642/661 nm, similar to Alexa Fluor™ 647 or allophycocyanin (APC)
  • This direct-to-sample dead-cell stain emits red fluorescence when bound to DNA
  • Add 2 drops form the dropper bottle directly into 1 x 106 cells, incubate, and analyze
  • No need to dilute, weigh, or pipette
  • Stable at room temperature
TRUSTED_SUSTAINABILITY

Specifications

Cell Permeability Impermeant
Color Far-red
Content And Storage Includes 2 dropper bottles, store at room temperature.
Description TO-PRO™3 Ready Flow™ Reagent
Detection Method Fluorescence
For Use With (Application) Viability Assay
For Use With (Equipment) Flow Cytometer
Product Type Reagent
Dye Type Cell-Impermeant
Emission 661
Excitation Wavelength Range 642 nm
Form Solution
Format Bottle(s)
Product Line Ready Flow, TO-PRO3
Quantity 120 assays
Solubility DMSO (Dimethylsulfoxide)
Sub Cellular Localization Nucleus
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Frequently Asked Questions (FAQs)

I would like to label only 100 µL of sample of the same cell density using a Ready Flow product. The manual notes 2 drops per 1 mL (1 X 10E6 cells/mL). May I scale down for smaller volumes?

Yes, you may scale up or down as needed, but we recommend keeping the cell density the same. There are 41 µL per drop, so 2 drops is 82 µL (for the 1 mL of sample). You need to only scale down for this 100 µL volume, which would be 8.2 µL.

What is the concentration of dye/reagent in the Ready Flow products?

The amount of dye or reagent in the Ready Flow products is proprietary. If you need to consider the exact amount of dye or reagent for your experiment, each of the Ready Flow reagents is available as a standalone product.
Can I use the ReadyProbes reagents for flow cytometry?

This is not recommended. The ReadyProbes reagents were developed for imaging applications whereas the Ready Flow reagents were optimized for flow cytometry.
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